FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110565
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76850.6919901131406085No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33900.30525003038993664No Hit
GCCTTGGGCTGACCTAGAACGGTCAGCTTGGTCCCTCCGCCGAACAGCAC27870.25095334356836385No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC26030.23438520032596022No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG25120.2261911729615106No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24100.2170066587727868No Hit
CCTTAGACCTCCCAGACCGATTCTCTGGCTCCTCCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGG22780.20512081688149725No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22620.20368010877346215No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18430.165951565194293No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTGACCATGTGCTGTTCGGCG16740.15073408580317224No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16660.1500137317491547No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16570.14920333343838496No Hit
CTCCAGCCCCTTCCCTGGTGCCTGGCGGACCCAACTCATAGCGTGGCGACCGAAGGTGAATCCAGAGGCCTCACA16430.14794271384385424No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16290.14668209424932355No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16230.14614182870881037No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16190.14578165168180163No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGAACGGTCAGCTTGGTCCCTC16130.14524138614128845No Hit
TTCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15980.14389072229000552No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15220.1370473587768388No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15210.13695731452008664No Hit
CTCCTCACTCTTTGCATAGGTTCTGTCGTTTCTTCTCAGCTGACTCAGGGCCCTGCTGTGTCTGTGGCCTTGGGA15180.13668718174983002No Hit
GACCTAGAACGGTCAGCTTGGTCCCTCCGCCGAACAGCACATGGTCACCA14980.13488629661478618No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14910.1342559868175208No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14770.1329953672229901No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTGACCATGTGCTGTTCGG14430.12993386249341551No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC14310.1288533314123892No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14110.1270524462773453No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13850.12471129560178829No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13820.1244411628315317No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13730.12363076452076197No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13600.12246018918298344No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13330.12002899425067422No Hit
GCATAGTACTTATAACTTCCACTGCTACTAACAGATGCGACCCACTCCAGCCCCTTCCCTGGTGCCTGGCGGACC13240.11921859593990447No Hit
GTTCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13080.11777788783186936No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12890.11606704695357767No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12670.11408607330502941No Hit
GGCCTGTTGGGTCCCTGAGACTCTCGTGTGAGGCCTCTGGATTCACCTTC12440.11201505539972897No Hit
CTCTTTGCATAGGTTCTGTCGTTTCTTCTCAGCTGACTCAGGGCCCTGCT12350.11120465708895923No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12250.1103042145214373No Hit
GGTCTAAGGAGCGGGAATTATTAGAATAGATGACAAGTAGAGGGGCCTGGCCTGGCTTCTGCTGATACCAGGTTG12210.10994403749442852No Hit
GTACTATGCAGACTCACTGAGGGGCCACCTCACCATCTCCAGAGACAACG12140.10931372769716315No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12080.10877346215665No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGGTGATCAGCACTGAGCACCGAGGATTCACCATGGAA12050.1085033293863934No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12030.10832324087288903No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11890.10706262127835832No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCC11880.10697257702160612No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11850.10670244425134955No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTAGCTATTTTAGAAGGT11710.10544182465681882No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11710.10544182465681882No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTCGTTTCTTCTCAGCTGACTCAGGG11560.10409116080553592No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11480.10337080675151837No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11130.1002192577651916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAT200.0079820350.69695315
GTGGTAT2600.044.1973421
TAACGGC955.456968E-1239.58027336
TTAACGG955.456968E-1239.52084735
AACGGCC901.3278623E-1038.04868337
GTATCAA20550.036.5116771
GCGGTAC1051.4551915E-1136.3759144
GTTTAAC1051.8189894E-1135.67256533
CGGTACC1102.5465852E-1134.79170245
CGCGGTA1003.619789E-1034.658343
GTATAAA601.5454218E-533.7979661
AATGGTA2204.838512E-1033.72625470
TAACCGT956.8121153E-933.55183853
TCGTATA1600.031.5837661
TTGCAAT950.00421919131.24116170
GTATTAG1550.030.5271971
ACCCTAA1401.2732926E-1130.05984349
CGGACAT450.00510593730.04940621
CGTCACT450.00510593730.04940620
TTACGGT450.005118324430.0345174
Produced by FastQC (version 0.11.5)