FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751632
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTTGGTAACTCACAGTACACTTTTGGCCAGGGGA37330.49665261723822296No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25900.34458351959469524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25670.34152351150563043No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21500.28604423441258486No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGGTACA17860.23761628030738446No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17780.23655192966770974No Hit
GTGTATTACTGTCAGCAGTTTGGTAACTCACAGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA17430.23189539561913278No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16370.21779274964344253No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16170.21513187304425568No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15390.20475445430742706No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14710.19570747387019177No Hit
GTGTATTACTGTGCAAGGGGAAGTGGGAACTACTACTTTGATTACTGGGGCCAGGGAACCCTGGTCACCGGCTC13880.18466483598356642No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13840.18413266066372908No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13220.17588394320624987No Hit
GTATTAATACTGATGGGAGTGGGACAAGGTCCGCGGACTCCGTGAAGGGCCGATTCACCGTATCCAGAGACAACG13040.17348915426698172No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTAGCTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG13000.17295697894714435No Hit
GAGTATTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG12800.1702961023479575No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12500.16630478744917726No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTACTTAGCCTGGTACC11870.15792302616173873No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT11840.1575238946718607No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11270.14994039636417822No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11250.14967430870425955No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11230.14940822104434084No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11100.14767865125486943No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10890.14488473082572323No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT10300.13703514485812207No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10270.13663601336824405No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC10130.13477339974881325No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10120.13464035591885393No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10100.13437426825893523No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9990.13291078612938245No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA9830.130782084850033No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAATACTCTGA9680.1287864274006429No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTTTGGTAACTCA9430.12546033165165932No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9400.1250612001617813No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG9280.1234646742022692No Hit
GTATTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCAT9240.12293249888243184No Hit
GGCCAAAAGTGTACTGTGAGTTACCAAACTGCTGACAGTAATACACTGCA9230.12279945505247249No Hit
GTATTAATACCTAAGACCCACACCAGCCCCTTCCCTGGAGCTTGGCGGAC9180.12213423590267578No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9120.12133597292291973No Hit
GTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTG9090.12093684143304169No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8620.11468378142495264No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC8430.11215594865572513No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG8410.11188986099580646No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8380.11149072950592843No Hit
AAAGTGTACTGTGAGTTACCAAACTGCTGACAGTAATACACTGCAAAATC8340.11095855418609107No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7800.10377418736828661No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7630.10151244225897779No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7530.10018200395938438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG150.002518114267.8460164
CCATATG200.006234817753.96330662
AACGGCA200.00752929651.4496736
GGTATCA7400.042.621621
GTATCAA20650.040.2369231
AACTGTG14150.039.24644570
ATCAACG23250.034.871393
TATCAAC23400.034.6409382
AACGCAG23850.034.136366
TCAACGC23850.033.9941254
CAACGCA24100.033.9230085
TAATAGT502.0546357E-433.9230044
TTTCGAT850.002994329433.50450570
CGGTACC851.1281554E-732.72267545
ACGCAGA24700.032.6869477
CGCAGAG25000.032.4303938
TAACGGC851.270746E-732.28214336
TTAACGG851.2812961E-732.2517535
CTGAAGG6700.031.87928870
AGAGTAC25900.031.16004811
Produced by FastQC (version 0.11.5)