FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016069
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC36160.355881342704088No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35970.3540113909586849No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT28350.2790164841167283No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGTGCTGCTTGTATATGAGCTGCAGTAGTAATCAG27030.2660252404118224No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26130.25716757424938663No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25480.2507703709098496No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGTCAACAAGG24280.23896014935993518No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT23760.23384238668830562No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22990.22626416119377718No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21870.2152412877471904No Hit
GATTACTACTGCAGCTCATATACAAGCAGCACCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG21740.21396184707928298No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18960.18660150048864793No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAAACATGTCTCCTGGTACCAACAAAACCCAGGCAAAGC18690.18394420063991718No Hit
TCTCTGGCCTCCAGGCTGAGGACGAGGCTGATTACTACTGCAGCTCATAT18420.18128690079118642No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18110.17823592689079185No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17980.17695648622288446No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17330.17055928288334748No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAAACATGTCTCCTGGTACCAACAAAA17090.1681972385733646No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17030.16760672749586888No Hit
GTTATAAACATGTCTCCTGGTACCAACAAAACCCAGGCAAAGCCCCCAAA16900.16632728682796147No Hit
GTAGTAATCAGCCTCGTCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG16900.16632728682796147No Hit
CACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16890.16622886831504552No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16360.1610126871305No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16310.16052059456592024No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15940.15687910958802995No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15610.15363129866180347No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT15320.15077716178724082No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15060.14821828045142604No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14870.1463483287060229No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGA14820.14585623614144314No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14700.1446752139864517No Hit
TATACAAGCAGCACCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCT14400.1417226585989731No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14370.14142740306022525No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14290.14064005495689763No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGTGCTGCTTGTATATGAGCTGCAGTAGTAATCAGC14020.13798275510816688No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13860.13640805890151161No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13730.1351286182336042No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13600.1338491775656968No Hit
GCTTGTATATGAGCTGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGGC13400.13188080730737772No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13140.12932192597156297No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12490.12292472263202597No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12210.12016900427037928No Hit
ATAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA11860.1167243563183209No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11580.1139686379566742No Hit
CCTCCAGGCTGAGGACGAGGCTGATTACTACTGCAGCTCATATACAAGCAGCACCGTGGTTTTCGGCGGAGGGAC11360.11180343067252321No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11290.11111450108211154No Hit
GCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11200.11022873446586796No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11140.10963822338837224No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11000.1082603642075489No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10570.1040283681521629No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10540.10373311261341503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGCG150.002352604969.0210441
TGCGCGG150.002448140668.3316537
GTACACG200.00810577250.4997183
TCTCGTA2400.044.2236238
TCGTATG2600.043.68281640
GGTATCA7650.042.7029231
CGAGACG2850.041.36515423
ATGCCGT3000.040.5506944
CGTATGC2850.039.95954541
ACGAGAC3050.039.75117522
ATCTCGT2700.039.2274337
TCTCCGA3250.038.3279911
ATCTCCG3300.037.74726510
CATCTCG2750.037.2123436
CTACATC2950.036.85143333
ACATCGA1001.3625114E-436.3747670
GCTACAT3200.036.056332
CTTATAC3400.035.659141
GTGGTAT2200.035.20891
TATACAC3650.035.050033
Produced by FastQC (version 0.11.5)