FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016069
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC32500.319860166976849No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32200.3169076115893704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30270.2979128385965914No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27630.27193035118677966No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGTGCTGCTTGTATATGAGCTGCAGTAGTAATCAG26440.2602185481497812No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT25060.2466367933673796No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG23840.23462973479163324No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGTCAACAAGG22310.21957170231549236No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT22070.21720965800550948No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20660.20333264768436No Hit
GATTACTACTGCAGCTCATATACAAGCAGCACCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG20580.2025452995810324No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20530.20205320701645263No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20010.19693544434482305No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19690.19378605193151252No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18680.18384578212700123No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAAACATGTCTCCTGGTACCAACAAAACCCAGGCAAAGC17880.17597230109372494No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17680.17400393083540588No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17410.17134663098667513No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17400.17124821247375915No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17280.1700671903187677No Hit
TCTCTGGCCTCCAGGCTGAGGACGAGGCTGATTACTACTGCAGCTCATAT17010.16740989047003696No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16600.1633747314404829No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAAACATGTCTCCTGGTACCAACAAAA16590.16327631292756695No Hit
GTTATAAACATGTCTCCTGGTACCAACAAAACCCAGGCAAAGCCCCCAAA16590.16327631292756695No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16040.1578632947171895No Hit
GTAGTAATCAGCCTCGTCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG15660.15412339122638324No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15440.1519581839422323No Hit
CACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15150.1491040470676696No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14960.1472340953222665No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14810.1457578176285272No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14770.14536414357686336No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14510.1428052622410486No Hit
GCTTGTATATGAGCTGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGGC13910.13690015146609139No Hit
TATACAAGCAGCACCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCT13860.13640805890151161No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGA13640.13424285161736063No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12890.12686146314866412No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12720.1251883484290929No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGTGCTGCTTGTATATGAGCTGCAGTAGTAATCAGC12610.12410574478701741No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11780.11593700821499328No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11750.11564175267624541No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11660.11475598606000184No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11510.11327970836626253No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11420.11239394175001893No Hit
CCTCCAGGCTGAGGACGAGGCTGATTACTACTGCAGCTCATATACAAGCAGCACCGTGGTTTTCGGCGGAGGGAC11220.11042557149169988No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11120.10944138636254035No Hit
ATAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA11090.10914613082379247No Hit
GCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11060.10885087528504463No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10770.10599673841048196No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10750.10579990138465005No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10720.1055046458459022No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10660.10491413476840647No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10650.10481571625549052No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10480.10314260153591931No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10250.10087897573885238No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10240.10078055722593643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.041.7752881
GTGGTAT2900.039.4049641
TGGTATC2950.035.3156282
GAGACGA502.1738629E-433.60019311
CCGCATA550.003181683133.096869
GTATCAA19550.032.492651
GTACATT500.0056114429.4741664
TGCGGCA754.043345E-529.36006463
GGAATCA1305.323861E-428.83544770
ATGCTAG1651.8189894E-1228.82378662
GATTGAC704.60187E-528.8086741
CAATAGA1050.00654084828.56082270
TATCAAC22250.028.5469072
CGCGCCA606.3317944E-427.99740412
CCAACGG656.6147343E-427.78860558
ATCAACG23250.027.6054573
TCAACGC23350.027.4912914
ATGCGGC806.4143016E-527.43802662
CAACGCA23500.027.3158155
AATGGTA3850.027.26260470
Produced by FastQC (version 0.11.5)