FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034050
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA57660.5576132682172042No Hit
GTGTATTACTGTGCGACATCCCCCCCGGGGGGCCGAGAATACTTCCAGCA36190.34998307625356606No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29970.28983124607127314No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26200.25337266089647503No Hit
GGAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA24120.23325757942072434No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23970.2318069725835308No Hit
GTATATTCCTGTCAGCACTATGGTAACTCATCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA23870.23083990135873508No Hit
CATCTATGCTGCTTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGACAGACTTCA20600.19921667230791548No Hit
CCCTAAGACTCTCGTGTGCAGCCTCTGGATTCACCATCAGTAGCAACTAC20170.19505826604129395No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19740.1908998597746724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19440.18799864610028527No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18970.18345341134374546No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA18940.18316328997630676No Hit
ATGGTAACTCATCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA17340.16769015037957546No Hit
GTATTACTGTGCGACATCCCCCCCGGGGGGCCGAGAATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGT16590.16043711619360765No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16510.1596634592137711No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16490.15947004496881195No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16450.15908321647889367No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16220.15685895266186353No Hit
GAGTTACCATAGTGCTGACAGGAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT16060.15531163870219042No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16060.15531163870219042No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16020.15492481021227214No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15420.1491223828634979No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA15310.14805860451622263No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15160.14660799767902907No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15030.14535080508679465No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14510.14032203471785698No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTCTCAGTTATTTATAGCGATGGTAGA14510.14032203471785698No Hit
GTCCCAGACCCACTGCCACGGAACCTGTCTGGGATGCCAGTGGCCCTGCT14260.1379043566558677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14140.13674387118611286No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14030.13568009283883758No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14010.13548667859387845No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGAAAACACTCTGA13940.13480972873652144No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTTTCAG13700.13248875779701175No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13560.13113485808229774No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGACGTGCAGCTGGTGGAGT13420.12978095836758377No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13300.1286204728978289No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12950.12523572361104396No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTTTCAGCAGCTACTTAGCCTGGTACC12930.1250423093660848No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12610.12194768144673857No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT12320.119143174894831No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12120.11720903244523959No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12090.11691891107780089No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12040.11643537546540304No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACGGAACCTGTCTGGGATGCCA11840.11450123301581161No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC11830.11440452589333205No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG11770.11382428315845462No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11650.11266379768869977No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11620.11237367632126107No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11460.11082636236158794No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTATA11380.11005270538175137No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT11330.1095691697693535No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11310.10937575552439438No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11230.10860209854455781No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11160.10792514868720081No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11020.10657124897248682No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11010.10647454185000725No Hit
ATATTCCTGTCAGCACTATGGTAACTCATCGTGGACGTTCGGCCAAGGGA10920.10560417774769111No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10870.10512064213529326No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10770.10415357091049755No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10620.10270296407330401No Hit
CTCCAGAGACAATGCCAAGAACACGCTGTATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTA10500.10154247860354915No Hit
GGGCTGGAGTGGGTCTCAGTTATTTATAGCGATGGTAGAACATACTATGC10500.10154247860354915No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10430.10086552874619215No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10430.10086552874619215No Hit
GCACTATGGTAACTCATCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT10360.10018857888883517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTA150.002115353570.8961455
TCTGGAG16650.060.39108370
TTATACA4650.048.0945472
CTTATAC4800.048.0243531
TATACAC4900.046.3322683
GACCGAT4800.045.2519326
GTATAGA306.8814366E-445.199391
ATCTCGT2200.045.19077337
TATCTCG2350.045.16206436
ACCGATC4900.044.34560827
TCGTATG2100.044.2922340
CGTATGC2750.043.92905841
GGTATCA9200.043.4798551
CCGATCA5100.043.29326628
ACGAGAC5050.042.96595822
GAGACCG5250.041.98508524
CGATCAG5250.041.44143729
ATGCCGT2600.041.36112644
AGACCGA5250.041.35316525
TCTCCGA5550.040.29542511
Produced by FastQC (version 0.11.5)