FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034050
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53330.5157390841835501No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA49560.479280499008752No Hit
GTGTATTACTGTGCGACATCCCCCCCGGGGGGCCGAGAATACTTCCAGCA37540.3630385377883081No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29520.28547942555969247No Hit
GTATATTCCTGTCAGCACTATGGTAACTCATCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA23450.22677820221459313No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22430.2169140757216769No Hit
GGAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA22400.2166239543542382No Hit
CATCTATGCTGCTTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGACAGACTTCA19530.18886901020260144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19260.18625791789565302No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18790.1817126831391132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18430.17823122672984865No Hit
CCCTAAGACTCTCGTGTGCAGCCTCTGGATTCACCATCAGTAGCAACTAC18060.17465306319810456No Hit
GTATTACTGTGCGACATCCCCCCCGGGGGGCCGAGAATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGT17760.17175184952371744No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17330.16759344325709588No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA16680.1613074802959238No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16490.15947004496881195No Hit
ATGGTAACTCATCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA16250.15714907402930225No Hit
GAGTTACCATAGTGCTGACAGGAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT15870.15347420337507858No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15820.15299066776268072No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15660.15144335380300758No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15530.15018616121077316No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA15470.14960591847589577No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTCTCAGTTATTTATAGCGATGGTAGA14450.13974179198297954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13990.1352932643489193No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13630.13181180793965475No Hit
GTCCCAGACCCACTGCCACGGAACCTGTCTGGGATGCCAGTGGCCCTGCT13570.13123156520477733No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13390.12949083700014508No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13370.1292974227551859No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTTTCAG13320.12881388714278807No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGACGTGCAGCTGGTGGAGT13190.12755669455055363No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13110.12678303757071707No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13070.1263962090807988No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13050.12620279483583965No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13020.12591267346840096No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGAAAACACTCTGA12770.12349499540641168No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12230.11827281079251487No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12190.11788598230259659No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTTTCAGCAGCTACTTAGCCTGGTACC12170.11769256805763745No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11890.11498476862820946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11860.11469464726077075No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11830.11440452589333205No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11550.11169672646390406No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTATA11500.11121319085150622No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT11410.11034282674919009No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACGGAACCTGTCTGGGATGCCA11240.10869880566703738No Hit
ATATTCCTGTCAGCACTATGGTAACTCATCGTGGACGTTCGGCCAAGGGA11000.1063778347275277No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG10930.1057008848701707No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10900.10541076350273197No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10820.10463710652289542No Hit
CTCCAGAGACAATGCCAAGAACACGCTGTATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTA10660.10308979256322227No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10590.10241284270586527No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10560.10212272133842658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG15750.045.43485370
AGGTACG306.9611333E-445.0938642
GGTATCA8800.044.583591
GTCACCG13300.043.2654270
ACGGCAG750.001361029939.34564670
GGTACGG350.001486906138.6481443
GTATCAA22750.038.207751
ATATACC400.0028635733.8171273
TTCGGAG450.003593505432.28544263
ATCAACG27350.031.7769723
TATCAAC27400.031.598622
TCAACGC27550.031.4220164
TTTCGAT950.004339104531.06235370
AACGCAG28200.030.5793156
CAACGCA28500.030.3746175
TATTGTA450.00509484930.0625762
GTTCCGA450.005097260230.059676
CGCAGAG29950.029.0169648
ACGCAGA29900.028.8406937
CATTGTC16400.028.79113263
Produced by FastQC (version 0.11.5)