FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207288
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA35760.29620107215511127No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34360.28460483331234965No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30980.25660819953482517No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26610.22041136828991922No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26550.21991438662522944No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC26150.21660117552729755No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAC24970.20682720278839845No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC24800.2054190880717774No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24440.2024371980836387No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23680.1961420969975681No Hit
GAAATATACGGCCGTGTCCGCGGCTCGTAGGCTGCTCATTAGAAGATACA22960.19017831702129068No Hit
CAGTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCCAGAGCTGGAGC22950.19009548674384238No Hit
GCCCCAGAGCTGGAGCCTGAAAAGCGATCAGGGATCCCGTCCCCCTTGCT22390.18545699120673775No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20910.17319811014438974No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT20510.16988489904645784No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTTTCAGGCTCCAGCTCTGGGGCTGAGCGCTCCCTCACCATCTCCAG20310.16822829349749188No Hit
CCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATCGG20250.16773131183280213No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT20200.1673171604455606No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20110.16657168794852595No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19510.16160187130162812No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATC19430.16093922908204172No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG19220.1591997932556275No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18900.15654922437728197No Hit
GTTCTTGATGAGGCTTAACAATGATGGCAGTCACAGCAAGGGGGACGGGATCCCTGATCGCTTTTCAGGCTCCAG18880.15638356382238539No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18750.15530677021555753No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18660.15456129771852284No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18410.1524905407823154No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA18340.1519107288401773No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18310.15166223800783243No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18290.15149657745293585No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17810.14752072413541756No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17610.14586411858645162No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17610.14586411858645162No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA17520.14511864608941694No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17460.14462166442472715No Hit
GCTCTGGGGCTGAGCGCTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACTGTCAGAC17240.14279939832086463No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17110.14172260471403675No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16930.1402316597199674No Hit
GTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGC16730.13857505417100147No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16680.13816090278375998No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16650.13791241195141507No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16400.13584165501520765No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16220.1343507100211383No Hit
CCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCACCCTCATCCTCA16060.13302542558196553No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15790.1307890080908615No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15670.12979504476148193No Hit
GTATATTTCTGTGCGAAAGCCCCCGCTCGAGGTGGCGTCGCTGACTACTGGGGCCAGGGAACCCTGGTCACCGT15420.1277242878252745No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14620.1210978656294107No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC14410.11935842980299648No Hit
CTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACT14380.11910993897065157No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14360.11894427841575499No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13990.11587955815016797No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13850.11471993426589182No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCCAGAGCTGGAGCCTGAAAAGCGATCAGGGATC13740.11380880121396054No Hit
GGCTTAACAATGATGGCAGTCACAGCAAGGGGGACGGGATCCCTGATCGC13610.11273200760713267No Hit
GGACTGGAGTGGGTCTCAGCTCTCAGTGGTAGTGGTGGTAGCACATACTA13560.11231785621989118No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG13420.11115823233561503No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13320.11032992956113206No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13310.11024709928368376No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13290.11008143872878716No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13190.10925313595430419No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT13150.10892181484451101No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13130.10875615428961442No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC13120.10867332401216612No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13100.1085076634572695No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13010.10776219096023484No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT12980.10751370012788994No Hit
CCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCC12950.10726520929554505No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGTTGC12890.10676822763085528No Hit
GCCCTGGGACTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA12840.10635407624361379No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT12740.10552577346913082No Hit
GGATGAGGGTGACTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT12650.10478030097209613No Hit
GTCTGAGGATGAGGGTGACTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAG12570.10411765875250975No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12560.10403482847506146No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12510.10362067708781998No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC12400.1027095440358887No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12360.10237822292609551No Hit
GCCTACGAGCCGCGGACACGGCCGTATATTTCTGTGCGAAAGCCCCCGCT12170.10080444765457786No Hit
GCACCTACGCCATCGCATGGCATCGGCAGCAGCCAGAGAAGGGCCCTCGG12120.10039029626733639No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12110.10030746598988809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4650.054.494670
CAACGAA200.00765611251.2347075
ATATCGT306.4076646E-445.8591537
CCTATCG306.471123E-445.7675334
TCGTATG4150.044.04841240
CGTATGC4350.043.65096341
GGTATCA11350.042.1592671
ATGCCGT4500.041.5365444
TATACAC5050.041.2583123
GAGACCG4900.041.14933424
CGAGACC4900.041.14251323
ATCTCGT3600.041.08215337
GCCGTCT4250.040.8077346
AGACCGG4950.040.74719225
ATCTCCG5150.040.4571810
ACGAGAC4900.040.4401622
CTTATAC5000.040.331361
TACACAT5350.040.2216385
GCCTATC4200.040.0116233
TATCTCG3700.039.93684436
Produced by FastQC (version 0.11.5)