FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353393
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA61940.4576645512426915No Hit
GTGTATTACTGTGCGAGAACGTCTTACTATGACAGTAGTGGTTATGGGCC42460.3137300104256487No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41770.308631713035312No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC40730.3009473227658189No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37300.2756036125500871No Hit
GTCTCTGGTGGCTCCATCAGAAATAGTTTTTACTTCTGGGGCTGGATCCG30790.22750228499777966No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT30730.22705895478992427No Hit
GCCTAGAGCCTGACGATTTTGCAGTTTATTACTGTCAGCAACGTAGCAAC28270.2088824162678542No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27670.2044491141893005No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26710.19735583086361463No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26140.19314419388898862No Hit
GTATTACTGTGCGAGAACGTCTTACTATGACAGTAGTGGTTATGGGCCTT25660.1895975522261457No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24860.18368648278807412No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA23780.1757065390466775No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG23710.17518932047084623No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA23660.17481987863096676No Hit
GTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCAGTGGCCCTGTT23420.1730465577995453No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23070.170460464920389No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22990.16986935797658184No Hit
GTTTATTACTGTCAGCAACGTAGCAACTTCGGCCCTGGGACCAAAGTGGA22370.16528827916207636No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22280.1646232838502933No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGACGATT22100.1632932932267272No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21750.16070720034757088No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCA21650.15996831666781194No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT20910.15450057743759574No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20720.15309669844605373No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20690.15287503334212607No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20630.15243170313427068No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20250.14962394515118668No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGAAATAGTTTTTACTTCTGGG20250.14962394515118668No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19850.1466684104321509No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19610.14489508960072942No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGACGATTTTGCAGTTTA19550.14445175939287405No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19540.14437787102489816No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19420.14349121060918743No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18950.1400184573143204No Hit
GTAATAAACTGCAAAATCGTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA18670.13794958301099533No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC18450.13632403891552564No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATG18210.13455071808410418No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18110.13381183440434521No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCAGTGG17450.1289352021179362No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17260.12753132312639417No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAAGGCCCATAACCACTACTGTCA16500.12191580716022618No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16040.11851694223333503No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16020.11836916549738324No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16010.11829527712940735No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15600.11526585404239567No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15340.11334475647502242No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15170.11208865421943219No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAGGCCCATAAC15070.11134977053967325No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14970.11061088685991431No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14920.11024144502003483No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14760.10905923113242051No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC14720.10876367766051694No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14290.10558647783755347No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14280.10551258946957756No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14190.10484759415779453No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14020.10359149190220432No Hit
GAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATGCATC13960.10314816169434894No Hit
CTGCTACTCTGGCTCCCAGTTTCAGTAACCACCGGAGAAATTGTGTTGAC13780.10181817107078285No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC13700.10122706412697569No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13690.10115317575899979No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGC13630.10070984555114443No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAAATAGTTTTTA13550.10011873860733726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT453.1008312E-853.5070939
TACAGAT18550.050.83812370
GGTATCA10550.044.1394921
CCGTCGT404.7144662E-543.5604447
TCGTATG6500.042.9286940
GTGGTAT2550.042.654911
ATCTCGT5600.042.2702937
TAGCGCT8550.042.25599329
TGGTATC2500.042.1359252
ACGAGAC8900.042.02176322
CGTATGC6900.042.0070841
CTTATAC9050.041.690171
CGAGACT8900.041.64436723
ATGCCGT7100.041.51693344
ACTAGCG8800.041.3937727
CTAGCGC8850.041.1827728
TTATACA9250.041.144062
GACTAGC8950.041.0606326
CGCTCAT7800.040.72142432
CACGAGA9250.040.42278721
Produced by FastQC (version 0.11.5)