FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353393
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87060.6432721315981389No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA57850.4274442087405506No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42440.31358223368969695No Hit
GTGTATTACTGTGCGAGAACGTCTTACTATGACAGTAGTGGTTATGGGCC42000.31033114549875757No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC37920.28018469136459256No Hit
GTCTCTGGTGGCTCCATCAGAAATAGTTTTTACTTCTGGGGCTGGATCCG30700.2268372896859966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30310.2239556433349367No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27090.20016358884669866No Hit
GCCTAGAGCCTGACGATTTTGCAGTTTATTACTGTCAGCAACGTAGCAAC26910.19883359822313254No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25830.19085365448173588No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25240.18649424077115812No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA23840.17614986925453285No Hit
GTATTACTGTGCGAGAACGTCTTACTATGACAGTAGTGGTTATGGGCCTT23090.17060824165634078No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG22640.16728326509742553No Hit
GTTTATTACTGTCAGCAACGTAGCAACTTCGGCCCTGGGACCAAAGTGGA22290.1646971722182692No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGACGATT21960.16225885607506468No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21670.16011609340376373No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21470.15863832604424585No Hit
GTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCAGTGGCCCTGTT21390.15804721910043867No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21330.1576038888925833No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT20680.15280114497415015No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCA20540.15176670782248763No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20480.15132337761463227No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20400.15073227067082512No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19980.14762895921583752No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA19820.14644674532822322No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19390.14326954550525975No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19340.14290010366538028No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19320.14275232692942846No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGACGATTTTGCAGTTTA19140.14142233630586237No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGAAATAGTTTTTACTTCTGGG18630.13765402953909175No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18520.1368412574913569No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18490.13661959238742924No Hit
GTAATAAACTGCAAAATCGTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA18080.13359016930041753No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCAGTGG17990.1329251739886345No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17750.13115185315721303No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17220.1272357696544906No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATG17040.1259057790309245No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC16600.12265469083998512No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16350.12080748164058777No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16270.12021637469678062No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15750.1163741795620341No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15670.11578307261822694No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15460.11423141689073314No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAGGCCCATAAC15300.11304920300311884No Hit
CTGCTACTCTGGCTCCCAGTTTCAGTAACCACCGGAGAAATTGTGTTGAC15180.1121625425874081No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14860.10979811481217946No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAAGGCCCATAACCACTACTGTCA14850.10972422644420357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14770.1091331195003964No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC14710.10868978929254103No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTC14650.10824645908468566No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14650.10824645908468566No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAAATAGTTTTTA14140.10447815231791505No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14010.10351760353422841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCAT200.005384334456.00360567
TACAGAT17900.049.56811570
CCGTGAG450.00951921248.4846670
GGTATCA11950.043.126761
CGTATCA350.001184494540.4785356
GTATCAA30850.038.906241
CGAACTA451.0787983E-437.85332529
GTATAAA451.1095804E-437.6728631
GTGGTAT3400.035.9000241
GGGACAA20450.034.4964270
CTATCTA400.002806503633.9570320
TGGTATC3600.032.967412
AACGCAG36900.032.7219546
TATCAAC36900.032.6228032
ATCAACG36900.032.5345153
CAACGCA37150.032.4104585
CTGGCGT13050.032.32310570
TCAACGC37150.032.2278634
CGCAGAG38350.031.4847478
ACGCAGA38500.031.4501767
Produced by FastQC (version 0.11.5)