FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119102
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52460.46876870919719565No Hit
CTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC47110.42096252173617776No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44540.39799768028294114No Hit
CTATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGG43170.385755721998531No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT42720.38173464081022107No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTC39690.3546593608089343No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG35450.3167718402790809No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34750.31051682509726547No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33040.2952367165816878No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31240.27915239182844814No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30710.2744164517622165No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28260.2525238986258625No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28210.2520771118271614No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG26270.2347417840375587No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25890.2313462043674303No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25260.22571669070379644No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA25150.224733759746654No Hit
ACCTTGATGTGACCCCACTTTCTAAACTGGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG24800.2216062521557463No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24620.21999781968042234No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24170.21597673849211244No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23050.20596871420120777No Hit
TCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT22990.20543257004276644No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAA22980.2053432126830262No Hit
GGCCAAAAGTGGCCGAGGGGGAATAACTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGG22600.20194763301289784No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22540.20141148885445653No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21870.19542454575186174No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21690.1938161132765378No Hit
GTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC21170.18916953057004632No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20930.18702495393628105No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT20900.18675688185706038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20600.18407616106485378No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA20110.179697650437583No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20080.17942957835836235No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG20000.17871471948044057No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19930.17808921796225902No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA19240.1719235601401838No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18590.1661153317570695No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17930.16021774601421496No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17850.1595028871362932No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17320.15476694707006156No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17060.1524436557168158No Hit
ACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC17000.15190751155837448No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16920.1511926526804527No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16860.1506565085220114No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA16770.1498522922843494No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16750.14967357756486896No Hit
CACTATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA16720.1494055054856483No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16720.1494055054856483No Hit
ATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16470.1471715714921428No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16290.14556313901681883No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16260.1452950669375982No Hit
GATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA15710.14038041215188607No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15680.1401123400726654No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15220.13600190152461528No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14320.12795973914799544No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC14290.12769166706877477No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13990.12501094627656817No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13810.12340251380124422No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13450.12018564885059628No Hit
CTTTCTAAACTGGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGG13390.11964950469215496No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13320.11902400317397342No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGTGACCCCA13280.11866657373501253No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAACACTATAGTAGTTATTCCCCCTCGGC13240.11830914429605166No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13170.1176836427778701No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13070.1167900691804679No Hit
GCCCCTAACCTCCTGATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGAT12960.11580713822332549No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12890.11518163670514395No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12880.11509227934540373No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12840.11473484990644285No Hit
AACTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT12790.11428806310774176No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA12570.11232220119345689No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12540.11205412911423625No Hit
CTATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCAC12520.11187541439475579No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12290.10982019512073071No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACAT12250.10946276568176985No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12050.10767561848696544No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT12040.10758626112722522No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11800.10544168449345993No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11730.1048161829752784No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11670.10428003881683706No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11610.10374389465839574No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11490.1026716063415131No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11290.1008844591467087No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11260.10061638706748803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)