FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119102
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGG53250.47582794061667305No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53130.4747556522997904No Hit
CTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC47070.4206050922972169No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT38690.34572362483491226No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTC38100.3404515406102393No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36190.3233842848998572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36080.3224013539427148No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35120.31382304740765365No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33440.2988110109712966No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG32380.2893391308388333No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30430.2719144456894903No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29840.26664236146481735No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29070.25976184476482034No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23750.21222372938302317No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23670.21150887050510142No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG23180.2071303598778306No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA23140.20677293043886974No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22960.2051644979635458No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22810.20382413756744247No Hit
GGCCAAAAGTGGCCGAGGGGGAATAACTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGG22320.19944562694017165No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22210.19846269598302926No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22150.19792655182458793No Hit
TCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT21880.19551390311160197No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAA21750.1943522574349791No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21730.19417354271549866No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20770.18559523618043752No Hit
ACCTTGATGTGACCCCACTTTCTAAACTGGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG20740.18532716410121688No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20680.18479101994277555No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20400.18228901387004937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20330.18166351235186784No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20040.17907214891940143No Hit
GTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC19850.17737435908433727No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19770.1766595002064155No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA19670.1757659266090133No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19590.17505106773109153No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG19370.1730852058168067No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18950.16933219670771743No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA18690.16700890535447172No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18380.16423882720252486No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17690.15807316938044969No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17420.15566052066746375No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17160.153337229314218No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGTGACCCCA16780.14994164964408963No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16400.14654606997396127No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16360.14618864053500039No Hit
ATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16030.14323984766357312No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15650.13984426799344474No Hit
ACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC15640.13975491063370452No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15500.13850390759734144No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA15490.1384145502376012No Hit
CACTATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA15100.13492961320773264No Hit
GATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA15090.13484025584799242No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14830.1325169644947467No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14720.13153403353760426No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14560.13010431578176074No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14520.12974688634279985No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC14330.12804909650773566No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14150.12644066403241172No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13990.12501094627656817No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13760.1229557270025431No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13720.12259829756358223No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13260.1184878590155321No Hit
CTTTCTAAACTGGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGG12830.11464549254670263No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12670.11321577479085909No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA12420.11098184079735358No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12350.11035633927917204No Hit
AACTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT12210.10910533624280898No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12040.10758626112722522No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11810.10553104185320017No Hit
GCCCCTAACCTCCTGATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGAT11810.10553104185320017No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAACACTATAGTAGTTATTCCCCCTCGGC11800.10544168449345993No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11680.1043693961765773No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11660.10419068145709687No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11610.10374389465839574No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11480.1025822489817729No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACAT11430.10213546218307179No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11400.10186739010385112No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11310.10106317386618913No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11290.1008844591467087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)