FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819240
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAA171450.9424265077724764No Hit
ATATAAGACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA70670.38845891691035817No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68690.37757525120379937No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55690.3061168399991205No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG47930.26346166531078913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45230.2486203029836635No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44180.2428486620786702No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAACTATATAAGACAGT41020.22547877135507136No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT40940.22503902728611946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39660.21800312218288959No Hit
GTTTATTACTGTCAGCAATATTTTGATATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA37750.20750423253666364No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA36770.20211736769200325No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34610.19024427783030276No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34380.18898001363206615No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33590.18463754095116644No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32680.1796354521668389No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC31190.17144521888261033No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29810.16385963369319057No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29750.16352982564147667No Hit
GTACAAGGCTGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACAACG29440.16182581737428817No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA29230.16067148919328952No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29010.15946219300367187No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28960.15918735296057693No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC28900.15885754490886306No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28630.1573734086761505No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27970.15374552010729756No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27860.15314087201248874No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT27220.14962291946087378No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26700.1467645830126866No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATT26310.14462083067654624No Hit
GCCTTGTACTTCTGCACGACCGGGGGAGTGGTTTGGGGAGTTAAAGTCTA24730.13593588531474682No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTCTTATATAGTTCCAC23870.1312086365735142No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23470.12900991622875488No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23000.12642641982366262No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGATGGATTTTC22600.12422769947890329No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22480.12356808337547548No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21970.12076471493590729No Hit
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTGCT21910.12043490688419341No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21780.1197203227721466No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21730.11944548272905169No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21690.11922561069457577No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21670.11911567467733777No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA21390.11757657043600625No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21270.11691695433257844No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA20920.11499307403091401No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20830.11449836195334315No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20600.11323409775510651No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20450.11240957762582177No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20280.11147512147929904No Hit
GGCAGGGGCAGCAAGATGGTGTTGCGGACCCAGGTCTTCATTTCTCTGTT19920.10949627316901563No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC19890.10933136914315868No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT19710.10834194498801697No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATTCCGTGGACGTT19340.10630812866911457No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC19310.10614322464325764No Hit
ACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTGCTCATT19200.10553857654844881No Hit
ATGCTGCACCCGTGAAAGGCAGATTCACCATCTCAAGAGATGATTCAAAA19060.10476902442778303No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTCTTATATAGTTCCACCAATAGGAACTA18980.10432928035883117No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18900.10388953628987928No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18840.1035597282381654No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18820.10344979222092741No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGATGGA18360.10092126382445417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.046.298061
CGCGTAT405.0526072E-543.0531839
GACGTAG5050.039.19857426
AGACGTA5050.039.1888725
CTTATAC5350.038.8801081
GTGGTAT2550.038.780131
CGAGACG5300.038.6151923
TCGTATG3850.038.50572240
TCGTTAA700.001516128438.49492670
ACGTAGA5100.038.15661227
TATACAC5350.037.596053
ACGAGAC5450.037.55135322
GAGACGT5400.037.2694524
CGTAGAG5250.037.07969328
AATCTCG3650.036.6824536
CGTATGC4150.036.59666441
TTATACA5700.035.8856122
ATCTCGT3500.035.34711537
CACGAGA5850.034.9827821
GCGTATG601.3493691E-534.47605540
Produced by FastQC (version 0.11.5)