FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760970
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134871.7723431935555936No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25190.3310248761449203No Hit
CCCCTATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCA24690.3244543148875777No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC24580.3230087914109623No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22640.297515013732473No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC21030.27635780648382985No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18190.2390370185421239No Hit
GCATAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC16770.22037662457127086No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16750.2201138021209772No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16530.21722275516774642No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15750.20697267960629195No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG15420.20263610917644584No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15340.20158481937527103No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG15040.1976424826208655No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14620.1921232111646977No Hit
GTACTTGATGAAGCTTATCAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG14260.18739240705941101No Hit
CAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG14200.1866039397085299No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13900.18266160295412434No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13760.18082184580206842No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC13660.1795077335505999No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG13640.1792449111003062No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT13070.1717544712669356No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12910.169651891664586No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC12750.16754931206223636No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT12730.16728648961194265No Hit
GGCATAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT12680.16662943348620837No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12350.16229286305636229No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12190.16019028345401262No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC11920.15664218037504765No Hit
GTAATATACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA11820.1553280681235791No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC11750.15440818954755114No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11630.15283125484578894No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11470.1507286752434393No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11290.14836327319079595No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC11230.14757480583991484No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT11100.14586645991300579No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11040.14507799256212467No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGTT10950.143895291535803No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10700.14061001090713168No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC10630.13969013233110372No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG10230.13443368332522965No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10230.13443368332522965No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC10100.13272533739832054No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA10040.13193687004743945No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9950.13075416902111778No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9850.12944005676964926No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC9670.1270746547170059No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9400.12352655163804092No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9280.1219496169362787No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9250.12155538326083815No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9250.12155538326083815No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCATAGGGGTGTTCGGCGGAGGGACCAAG9180.12063550468481017No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC8950.11761304650643259No Hit
GCTTATCAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCT8940.11748163528128572No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG8630.1134078873017333No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8620.11327647607658646No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8490.11156813014967738No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT8410.11051684034850257No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC8400.11038542912335572No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8180.10749438217012497No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8090.10631168114380331No Hit
GTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG8020.10539180256777533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG150.002668771266.858913
CGCCGTA200.00620946154.01891355
CTCGTTG200.00798070250.6974931
TCTGGAG4450.049.3311470
GACAGGT1600.041.7813261
GTATCAA24500.038.745781
CGATAAG350.001542550438.35893227
ATGTAAT451.2063276E-437.1389541
GGTATCA9400.036.9809151
GTGGTAT3150.036.0778431
TCTCGGT400.002452946534.9002745
GATCTCG400.002509620534.73813243
TTCGGCT4900.034.13862219
CGTGTCT4850.033.77186613
GACGATA502.2161075E-433.488925
GAATTGT4950.033.47611262
TCGGCTC5300.033.4580520
CGGCTCT5150.033.13757721
TATCAAC28750.032.6758042
TACGGCC5350.032.498597
Produced by FastQC (version 0.11.5)