FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102495
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52410.4753763055614765No Hit
GTGATATACGGCCGTATCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA35650.32335747554410676No Hit
GATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC31440.2851713613213665No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27220.24689454373942737No Hit
GAGTATTACTGCCTGCTCTACTATGGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG26550.24081741867310055No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCC26380.23927546156671914No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25640.23256341298599995No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC24040.21805087551417468No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21600.19591925586964115No Hit
GTATTACTGCCTGCTCTACTATGGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC21530.19528433235524878No Hit
GTATATCACTGTGTGAAGCACCTCTATTACTATGATAGTAGTGGTGACTT20710.18784665690093832No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20000.18140671839781586No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG20000.18140671839781586No Hit
CTACTATGGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC19420.17614592356427922No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA18300.16598714733400152No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18030.16353815663563098No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC17050.154649227434138No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17040.1545585240749391No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16830.15265375353176205No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG16590.15047687291098827No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT16420.14893491580460683No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16230.14721155197982758No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16220.14712084862062866No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16160.14657662846543523No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACCACCATAGTAGAGCAGGCAGTAATACTCAG16120.1462138150286396No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15960.14476256128145704No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15900.1442183411262636No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15660.14204146050548982No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC15610.1415879437094953No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15570.14122513027269964No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15470.14031809668071057No Hit
GTCCAGGAGTGTTTGTTGCTTGTACTATAAATCAGTGCCCTGGGTGCTTG15390.1395924698071193No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15310.13886684293352805No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14410.13070354060562633No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14200.12879877006244927No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14090.1278010331112613No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13980.1268032961600733No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13340.12099828117134317No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12990.1178236635993814No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12880.11682592664819343No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12860.1166445199297956No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG12850.1165538165705967No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC12590.11419552923142508No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12540.11374201243543054No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12490.113288495639436No Hit
CTATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATAGTAATCACTGGTGACTGCT12470.11310708892103818No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC12010.10893473439788842No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11930.10820910752429716No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11760.10666715041791573No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11750.10657644705871683No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11400.10340182948675504No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11400.10340182948675504No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11310.10258549925396486No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11220.1017691690211747No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11140.10104354214758343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG12300.055.7690270
ACGCGAA200.007132579652.16045445
GTGGTAT3000.044.9951741
GGTATCA11500.043.136681
AGGACTA709.2006696E-442.60614870
TGGTATC3350.041.301542
GTATCAA28700.037.391461
ACCGCAG502.0507042E-433.93579529
ATACCGC400.002841439833.87087627
ATTGTCT12500.032.9919464
AATTGTC12750.031.93030763
GAATTGT13050.031.62505562
TATCAAC33900.031.556352
TCTCTGG13800.031.32082468
ATCAACG34700.030.8358213
TCAACGC34850.030.7086754
GGAATTG13450.030.60705461
TCCGAGC603.783316E-430.57195765
AACGCAG35250.030.4559826
GAGCGAC1151.5352271E-930.29460746
Produced by FastQC (version 0.11.5)