FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156574
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45810.3960836055453434No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42090.3639196454355709No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA38010.3286430440248527No Hit
CTATTACTGCATGCAATCTCTACAAACTTTGATCACCTTCGGCCAAGGGA34270.296306159398361No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32830.2838555941945781No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA32500.2810023396687112No Hit
GTAATAGACAGCCGTATCTTCAACTCTCAGGCTGTTCATTTGCAGATACA32250.2788407832097211No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32240.27875432095136154No Hit
CCCTTGGAGAGCCGACCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG30260.26163479379616006No Hit
GTAATGGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT29610.2560147470027858No Hit
GGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACT26000.22480187173496896No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25960.22445602270153053No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC25520.220651683333708No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTA24730.21382116492329933No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24690.2134753158898609No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24240.20958451426367877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24240.20958451426367877No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23810.2058666371542158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23800.2057801748958562No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23590.20396446747030453No Hit
GTCTATTACTGCATGCAATCTCTACAAACTTTGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA23260.2011112129444376No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23000.19886319422708792No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22510.19462654356746736No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21980.19004404387440837No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC21910.18943880806589114No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21710.18770956289869908No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA20830.18010088416305398No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20650.1785445635125811No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19990.1728380544608473No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT19800.17119527155201483No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA18960.16393244184980815No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT18870.1631542815245717No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG18560.160473951515424No Hit
GTTGTATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGTCGGCTCTCCAAGGGTGACGGG18300.15822593279807431No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18060.15615083859744383No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGCCC17890.15468098020533055No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17490.15122248987094644No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGAGAGCCGACCTCCATCTCCT16930.14638060340280865No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16840.14560244307757222No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC16810.1453430563024934No Hit
CAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGCC16760.1449107450106954No Hit
CTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC16610.14361381113530133No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16480.14248980177662648No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16430.14205749048482846No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15920.1376479153084887No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15820.13678329272489265No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15680.13557282110785823No Hit
GTCAGGGACCCCGGAGGGCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA15620.1350540475577006No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15520.13418942497410455No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG15170.13116324593151843No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA14960.12934753850596675No Hit
CTACAAACTTTGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG14530.1256296613965038No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG14310.12372749171259254No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14290.12355456719587332No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13930.12044192589492761No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13840.11966376556969116No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGTC13410.11594588846022821No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13300.11499480361827259No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13270.11473541684319379No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13250.11456249232647457No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT13170.11387079425959774No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13150.11369786974287854No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12970.11214154909240567No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12630.10920183230817915No Hit
GCATAGTAATGGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGC12620.10911537004981955No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCAAAGTTTGTAGAGATTGCATGCAGTAATAGACCCCAACATCCTCAGCCT12620.10911537004981955No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12360.10686735133246987No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12230.10574334197379501No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12210.10557041745707582No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12200.1054839551987162No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG12170.1052245684236374No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12140.10496518164855859No Hit
GAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAATCTCTACAAACTTTGATCACCTTCGGCCAAGGGA11950.10332239873972612No Hit
GACTAGACCTGCAGGAGATGGAGGTCGGCTCTCCAAGGGTGACGGGCAGG11780.10185254034761285No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11760.10167961583089366No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAATCTCTA11700.10116084228073603No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11650.10072853098893801No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11570.10003683292206118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT353.3300785E-759.03196339
ATCGCGT554.3655746E-1156.2209637
TCTGGAG9450.054.47681470
GTATGCC5000.053.921742
ATTGACG200.006970647352.4644550
TCGTATG5000.052.41266640
CGTATGC5050.051.2827241
ATGCCGT4800.051.24004744
TAACGAC200.00770225151.15675425
TGTCGTA950.050.69804438
TCGACGT7150.050.16168229
CTCGACG7150.050.1356128
CGACGTC7250.049.49768430
ATCTCGT4250.049.3127637
CTTATAC7300.048.564121
GTCGTAT1000.048.20943539
CCGTCTT4700.048.11099647
TGCCGTC5150.047.82708445
ACTCGAC7500.047.77318227
TCCGAGC7600.047.5256813
Produced by FastQC (version 0.11.5)