FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156574
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65840.5692675090396292No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47480.4105228026913972No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA36720.31748941269646386No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA32010.2767656890090906No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31520.27252903834947007No Hit
CTATTACTGCATGCAATCTCTACAAACTTTGATCACCTTCGGCCAAGGGA31110.26898408575672633No Hit
GTAATAGACAGCCGTATCTTCAACTCTCAGGCTGTTCATTTGCAGATACA30770.26604436897249983No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30650.26500682187218455No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29540.2554095111942686No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28990.2506540869844904No Hit
GTAATGGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT27860.24088385178985522No Hit
CCCTTGGAGAGCCGACCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG26690.23076776756178163No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24820.21459932524853578No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC24730.21382116492329933No Hit
GTCTATTACTGCATGCAATCTCTACAAACTTTGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA23690.20482909005390057No Hit
GGATACAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACT23640.20439677876210255No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23350.20188937326967404No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22840.19747979809333427No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22630.19566409066778262No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22610.1954911661510634No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22000.1902169683911276No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTA21600.18675847805674345No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC20150.17422145059460095No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG19860.17171404510217245No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19820.17136819606873405No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19750.1707629602602168No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19240.16635338508387704No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA19050.16471060217504457No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19030.16453767765832536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19010.16436475314160615No Hit
GTTGTATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGTCGGCTCTCCAAGGGTGACGGG18140.15684253666432066No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT18080.15632376311416304No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17980.155459140530567No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17570.15191418793782327No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17430.1507037163207888No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC17310.14966616922047357No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17190.14862862212015834No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGAGAGCCGACCTCCATCTCCT16670.14413258468545895No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16650.14395966016873973No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16370.14153871693467085No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16340.14127933015959204No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA16320.14110640564287283No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGCCC16240.140414707575996No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15550.13444881174918336No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15040.13003923657284358No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14820.1281370668889323No Hit
CTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC14780.1277912178554939No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14550.12580258591322302No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG14170.1225170200955581No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG14110.12199824654540047No Hit
GTCAGGGACCCCGGAGGGCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA14100.12191178428704087No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA14010.12113362396180442No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13910.1202690013782084No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13820.11949084105297197No Hit
CAACTATGTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGCC13770.11905852976117395No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT13400.11585942620186862No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13220.11430310555139576No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13200.11413018103467655No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13130.11352494522615933No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13060.1129197094176421No Hit
CTCCCTGCCCGTCACCCTTGGAGAGCCGACCTCCATCTCCTGCAGGTCTA12940.11188216231732687No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12880.11136338876716924No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12860.11119046425045002No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12720.1099799926334156No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12680.10963414359997717No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12640.10928829456653877No Hit
GGCCGAAGGTGATCAAAGTTTGTAGAGATTGCATGCAGTAATAGACCCCA12470.1078184361744255No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCAAAGTTTGTAGAGATTGCATGCAGTAATAGACCCCAACATCCTCAGCCT12340.10669442681575066No Hit
GAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAATCTCTACAAACTTTGATCACCTTCGGCCAAGGGA12300.10634857778231224No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12130.10487871939019898No Hit
CTACAAACTTTGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG12060.10427348358168177No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11900.1028900874479281No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11870.1026307006728493No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11660.10081499324729763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATA200.00644179853.52157260
TCTGGAG8250.053.32451670
GTGGTAT2850.052.4538351
TAGCCGC200.00781692150.9658747
TGGTATC3300.044.2695542
GGTATCA11250.039.562911
GTATCAA30100.039.0552181
TATCAAC33600.034.6821062
CTCGCTA601.47600185E-534.02581423
ATCAACG34950.033.2513
TCAACGC35100.032.720254
ATTGTCT8650.032.575864
CAACGCA35950.031.945235
AACGCAG36000.031.900866
AGTGTTC8500.031.80564352
AATTGTC8850.031.31047463
GAATTGT8850.031.19187462
ACGCAGA37200.030.8718017
AGAGTAC38250.030.81714211
CGCAGAG37950.030.4407488
Produced by FastQC (version 0.11.5)