FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801266
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACACCTCCAACAATAAGAGCTACTTAGCTTGGTATCAGCAGAG84421.053582705368754No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATATGGGGTCCCT48830.6094106077132937No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT43660.5448877151907107No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40500.5054501251769076No Hit
GTTTAGGAGACTGTCCTCCTCTCTGCTGATACCAAGCTAAGTAGCTCTTATTGTTGGAGGTGTATAGAACAGT39250.48984981267144745No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35480.4427992701549798No Hit
GTATAGAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA35270.44017841765406246No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27480.3429572701200351No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25930.3236128826132645No Hit
GAGGAGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAA23750.29640593760374206No Hit
GTTTATTACTGTCACCAATATTTAATAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA22270.2779351675972773No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22150.27643753759675316No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21310.26595412759308396No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21080.2630836700920793No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20680.25809157009033207No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20060.25035381508762383No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19920.24860658008701234No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19320.24111843008439146No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18780.23437909508203267No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18740.23387988508185797No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18120.22614213007914977No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTTAATAACT17770.22177404257762093No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17520.21865398007652892No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT16970.21178984257412642No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16500.20592412507207344No Hit
GGAATATGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCCGGGACAGACT14850.18533171256486608No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14300.17846757506246366No Hit
GTCCTCCTCTCTGCTGATACCAAGCTAAGTAGCTCTTATTGTTGGAGGTG14150.17659553756180843No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTCTATACACCTCCAA14070.175597117561459No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTCTATA14040.17522271006132795No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCGGACCCGCTGCCA14010.1748483025611969No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13580.16948179505931862No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13290.1658625225580519No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13160.16424009005748405No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13120.1637408800573093No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12730.15887358255560574No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12670.1581247675553437No Hit
TAATAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA12620.15750075505512526No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12440.155254310054339No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12080.1507614200527665No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC11960.14926379005224233No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11770.1468925425514124No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11760.1467677400513687No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11380.14202524504970884No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11280.14077722004927204No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11160.13927959004874785No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10790.13466189754713168No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10540.13154183504603964No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10530.13141703254599596No Hit
CCATATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGACTGTCC10490.13091782254582124No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10490.13091782254582124No Hit
CAATAAGAGCTACTTAGCTTGGTATCAGCAGAGAGGAGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTAC10460.1305434150456902No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10460.1305434150456902No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10390.12966979754538444No Hit
ATTCAGTGGCAGCGGGTCCGGGACAGACTTCACTCTCACCATCAGCAGCC10320.12879618004507867No Hit
CTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC10120.12630013004420504No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10070.12567611754398664No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT9910.12367927754328774No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9900.12355447504324407No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9890.12342967254320039No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCCGGGACAGACTTCACTCTCAC9810.12243125254285095No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9750.12168243754258885No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9660.12055921504219573No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9620.12006000504202101No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9540.11906158504167155No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9520.11881198004158419No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTCTATACACCTCCAACAATAAGAGCTA9510.11868717754154053No Hit
GTAAATGAGCAGTTTAGGAGACTGTCCTCCTCTCTGCTGATACCAAGCTA9510.11868717754154053No Hit
GTCCGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG9430.11768875754119106No Hit
GCTCATTTACTGGGCATCTACCCGGGAATATGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCCGGGACAGACTT9370.11693994254092899No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9320.11631593004071059No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9260.11556711504044849No Hit
GAGTTATTAAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGT9240.11531751004036112No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9170.11444389254005537No Hit
GTCAGGGACCCCATATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGACTGTCCTCCTCTCTGCTGATA9150.114194287539968No Hit
GCAGAGAGGAGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATATGGGGTCCCTGACCGATT8960.11182304003913807No Hit
ATATTTAATAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8950.11169823753909439No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTTAATAACTCCGTGGACGTT8930.11144863253900701No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC8920.11132383003896335No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCGGACCCGCTGCCA8860.11057501503870125No Hit
TTATTACTGTCACCAATATTTAATAACTCCGTGGACGTTCGGCCAAGGGA8790.10970139753839547No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8760.10932699003826446No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8750.10920218753822075No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8740.10907738503817709No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8720.10882778003808974No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC8720.10882778003808974No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTTAATAACTCCGT8610.10745495253760924No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8560.10683094003739083No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG8550.10670613753734715No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8480.10583252003704138No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8460.10558291503695402No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG8300.10358607503625511No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8290.10346127253621144No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8270.1032116675361241No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8230.10271245753594938No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8140.10158923503555622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT150.002150178270.603247
CGTTTGC308.82281E-657.82569541
CCGTATT252.2912808E-456.4825647
GCCGTAT252.3329501E-456.2769446
AAGGGGG11350.051.66567669
AAAGGGG9950.051.49218868
TTAGCGC200.00860305649.7436648
TCGTATG12200.049.58670840
ATGCCGT12100.049.4293244
GCGATCT7750.049.40433534
CCGTCTT11000.049.1013247
CGTCTTA651.6370905E-1049.06165748
CGTATGC12700.048.90141341
CGATCTC7900.048.56847835
ATCTCGT7750.048.5134237
CTTGAAA11550.048.4897657
GATCTCG7800.048.4629536
GTATGCC12950.048.33258442
CGTCTTC10750.048.12352848
TGCTTGA10900.047.99524355
Produced by FastQC (version 0.11.5)