FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801266
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141691.7683266231189143No Hit
GTTCTATACACCTCCAACAATAAGAGCTACTTAGCTTGGTATCAGCAGAG68630.856519557799782No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40690.5078213726777375No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATATGGGGTCCCT39070.4876033676706612No Hit
GTTTAGGAGACTGTCCTCCTCTCTGCTGATACCAAGCTAAGTAGCTCTTATTGTTGGAGGTGTATAGAACAGT34880.43531112015235895No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27200.33946280011881197No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25230.31487670761020686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25150.3138782876098574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23820.29727955510404785No Hit
GTATAGAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA23680.2955323201034363No Hit
GTTTATTACTGTCACCAATATTTAATAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA23220.289791405101427No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCGGACCCGCTGCCA23070.28791936760077175No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20520.2560947300896332No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19160.23912159008369255No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19030.23749915758312473No Hit
GAGGAGGACAGTCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAA18870.23550231758242582No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18660.23288146508150853No Hit
GAGTGAAGTCTGTCCCGGACCCGCTGCCACTGAATCGGTCAGGGACCCCA18100.22589252507906238No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17790.22202364757770826No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTTAATAACT17490.21827957257639785No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17210.2147851025751748No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17090.2132874725746506No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16760.20916899007320916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16400.20467610007163664No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15920.19868558006953993No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15770.19681354256888473No Hit
GTCCCGGACCCGCTGCCACTGAATCGGTCAGGGACCCCATATTCCCGGGT15190.18957499756635124No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14880.18570612006499715No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14820.18495730506473507No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14790.18458289756460403No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14110.1760963275616337No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13460.16798416505879446No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12990.16211844755674146No Hit
GAGTTATTAAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGT12910.16112002755639201No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12780.15949759505582414No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTCTATA12610.15737595255508158No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCGGACCCGCTGCCA12590.15712634755499422No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTCTATACACCTCCAA12530.15637753255473213No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12530.15637753255473213No Hit
TAATAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA12400.1547551000541643No Hit
GTCCTCCTCTCTGCTGATACCAAGCTAAGTAGCTCTTATTGTTGGAGGTG12280.1532574700536401No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12270.15313266755359645No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCGGACCCGCTGCCACTGAATCGGTCAGGGACC12250.15288306255350906No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11920.1487645800520676No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11770.1468925425514124No Hit
GGAATATGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCCGGGACAGACT11490.14339807255018933No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11430.14264925754992724No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11060.13803156504831104No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10800.13478670004717536No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10580.13204104504621436No Hit
CTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC10540.13154183504603964No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10420.13004420504551548No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10180.12704894504446712No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTTAATAACTCCGT10170.12692414254442344No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10060.12555131504394296No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT9900.12355447504324407No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9850.12293046254302567No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9780.12205684504271991No Hit
GTCCGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG9700.12105842504237045No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9440.11781356004123475No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC9390.11718954754101633No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9330.11644073254075427No Hit
ATTCAGTGGCAGCGGGTCCGGGACAGACTTCACTCTCACCATCAGCAGCC9320.11631593004071059No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9310.11619112754066689No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG9250.11544231254040482No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9250.11544231254040482No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9250.11544231254040482No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9150.114194287539968No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8990.11219744753926911No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8960.11182304003913807No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8910.11119902753891966No Hit
CCATATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGACTGTCC8850.11045021253865757No Hit
GTAAATGAGCAGTTTAGGAGACTGTCCTCCTCTCTGCTGATACCAAGCTA8840.1103254100386139No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG8810.10995100253848285No Hit
TTATTACTGTCACCAATATTTAATAACTCCGTGGACGTTCGGCCAAGGGA8730.1089525825381334No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8650.10795416253778396No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGTTATTAAATATTGGTGACAGTAATAAACTGCCACATCTTCAGC8600.10733015003756555No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8570.10695574253743452No Hit
ATATTTAATAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8560.10683094003739083No Hit
GTCAGGGACCCCATATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGACTGTCCTCCTCTCTGCTGATA8470.1057077175369977No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8390.10470929753664826No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8370.10445969253656089No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8370.10445969253656089No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8330.10396048253638616No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTTAATAACTCCGTGGACGTT8250.10296206253603672No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8210.102462852535862No Hit
ATTAAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTC8110.10121482753542518No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCCGGGACAGACTTCACTCTCAC8060.10059081503520678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)