FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893188
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTAATCGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT35160.39364613048988567No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34870.3903993336229327No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31930.35748353090278867No Hit
GTATTATAGTGGGAGCACGTACCACAACCCTTCCCTCAAGAGTCGAGTCACCATATCGGTAGACACGTCCAAGAA25580.2863898753677837No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTC22810.2553773673627501No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22460.25145881941987575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21040.23556071062307152No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20320.22749969771201584No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACTACTGGGGCTGGATCCG18190.20365253451680945No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17990.20141336426373843No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17710.198278525909439No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17540.19637523119432865No Hit
CTATAATACATACTCCCAATCCACTCCAGCCCCCTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACGACTA17420.19503172904248603No Hit
GTTTATTACTGTCAGCAATATTATAGTAATCGGGCGTTCGGCCAAGGGAC17330.19402410242860404No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17260.1932403928400292No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17180.1923447247388008No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17020.19055338853634396No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16180.1811488734734457No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15920.17823795214445334No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15890.1779020766064927No Hit
GAAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA15280.1710726073346261No Hit
AACCAGGACAGCCTCCTAAGTTGCTAATTTACTGGGCATCTACCCGGGAA15230.17051281477135832No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15220.17040085625870477No Hit
GTTTTATCCAACTCCAACGATAGGAACTACTTAGCTTGGTACCAGCAGAA14300.16010067309457807No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14260.15965283904396388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13330.14924069736718362No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACTACTGGG13300.14890482182922296No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATCCAACTCCAA13140.14711348562676613No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13040.14599390050023064No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12910.14453843983573447No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC12530.14028401635489954No Hit
GATTTCACTCTCACCATCGGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAAT12500.13994814081693888No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATC12450.1393883482536711No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12100.13546980031079683No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGC11980.13412629815895422No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11980.13412629815895422No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11960.13390238113364714No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGC11630.13020775021607991No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11570.12953599914015862No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11410.1277446629377018No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCGTTGGAGTTG11290.1264011607858592No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11290.1264011607858592No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11260.12606528524789853No Hit
ATGTATTTCTGTGCGAGAGAGACGGCTACAATTGGGTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT11160.12494570012136304No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCGTTGGAGTTGGATAAAACACT10810.12102715217848874No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTAATTTACTGGGCATCTACCCGGGAATC10790.12080323515318164No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATTAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA10780.12069127664052809No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10510.1176683967988822No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10490.11744447977357511No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10490.11744447977357511No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10310.11542922654581118No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10130.11341397331804727No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCGGCAGCC9850.11027913496374783No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT9820.10994325942578718No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9810.10983130091313364No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9720.10882367429925167No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9550.1069203795841413No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9410.10535296040699159No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA9400.10524100189433805No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9340.10456925081841673No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9260.10367358271718832No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAATCGGG9130.10221812205269216No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9120.1021061635400386No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9120.1021061635400386No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATCCAACTCCAACGATAGGAACTA9080.1016583294894244No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9060.1014344124641173No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9020.1009865784135031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT252.582586E-455.13179439
TCGGCTT200.006902995552.59298753
GTGGTAT2150.046.0297551
CGCTCAT2600.044.80882632
TGGTATC2200.043.422742
TAGCGCT3000.043.31538829
CTAGCGC2950.042.8759128
ACTAGCG2950.042.8542827
GCGCTCA3050.042.66275431
ATCTCGT2350.040.9606437
CTTATAC3250.040.950611
TCGTATG2200.040.76844840
CGAGACT3100.040.73957423
GTATCAA19000.040.5913961
CACGAGA2950.040.49242821
CGTATGC2400.040.2959541
ACGAGAC3050.040.28605722
GGTATCA6750.039.9394871
CATCTCG2500.039.85787236
GACTAGC3200.039.49964526
Produced by FastQC (version 0.11.5)