FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893188
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTAATCGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT35290.3951015911543818No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33830.3787556483069634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30900.3459518040994729No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTC30560.3421452146692522No Hit
GTATTATAGTGGGAGCACGTACCACAACCCTTCCCTCAAGAGTCGAGTCACCATATCGGTAGACACGTCCAAGAA25820.28907687967146894No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23440.2624307536599238No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22170.2482120225529228No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22040.24675656188842665No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACTACTGGGGCTGGATCCG18720.20958633568744764No Hit
CTATAATACATACTCCCAATCCACTCCAGCCCCCTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACGACTA18110.20275686641558105No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18080.2024209908776204No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17950.20096553021312422No Hit
GTTTATTACTGTCAGCAATATTATAGTAATCGGGCGTTCGGCCAAGGGAC17550.19648718970698217No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17090.1913370981249188No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16210.18148474901140632No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16110.1803651638848708No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16100.18025320537221726No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14810.1658105572399092No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14280.159876756069271No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14270.15976479755661743No Hit
GAAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA14160.15853325391742837No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13590.15215161869617597No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13410.15013636546841203No Hit
GATTTCACTCTCACCATCGGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAAT13030.14588194198757706No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12890.14431452281042736No Hit
TCCTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12800.1433068961965454No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12800.1433068961965454No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACTACTGGG12750.14274710363327764No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12710.14229926958266345No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12580.14084380891816728No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12510.14006009932959243No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12450.1393883482536711No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12160.13614155138671813No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT11660.13054362575404058No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG11510.12886424806423732No Hit
AACCAGGACAGCCTCCTAAGTTGCTAATTTACTGGGCATCTACCCGGGAA11390.1275207459123947No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11290.1264011607858592No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11210.1255054926846308No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11100.12427394904544171No Hit
ATGTATTTCTGTGCGAGAGAGACGGCTACAATTGGGTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT10760.120467359615221No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10730.12013148407726032No Hit
GTTTTATCCAACTCCAACGATAGGAACTACTTAGCTTGGTACCAGCAGAA10720.12001952556460677No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATC10290.11520530952050408No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10120.11330201480539372No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10000.1119585126535511No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATCCAACTCCAA9950.11139872009028336No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9930.11117480306497624No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9930.11117480306497624No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9880.11061501050170848No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATTAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA9830.11005521793844074No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9730.10893563281190521No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCGTTGGAGTTG9600.10748017214740906No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9600.10748017214740906No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9550.1069203795841413No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9490.10624862850821999No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAATCGGG9390.10512904338168447No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCGTTGGAGTTGGATAAAACACT9340.10456925081841673No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9340.10456925081841673No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9310.10423337528045608No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9290.10400945825514898No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9260.10367358271718832No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9140.10233008056534572No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9010.10087461990084955No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9000.10076266138819599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)