FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504524
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52810.35100802645886675No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44110.293182428462424No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA40800.2711821147419383No Hit
GTGTATCACTGTCAGCAGTATGCTAGCTCAATCACTTTCGGCGGAGGGAC39370.26167744748505173No Hit
GTAATACATAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGATACA38380.25509729323028413No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35420.23542329667057488No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33550.22299411641156938No Hit
GTATCACTGTCAGCAGTATGCTAGCTCAATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG33390.22193065713807156No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAAGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA32630.21687922558895706No Hit
CTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA31050.2063775652631663No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30870.20518117358048127No Hit
GTCTCCAGACACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCT30830.2049153087621068No Hit
GTGATACACTGCAGAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30510.20278839021511125No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATT30310.20145906612323897No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTAACTATTTAGCCTGGTACC28250.18776702797695483No Hit
ATGTATTACTGTGCGAGAGATGGAATAGCAGTGTATACTGGGGCTGCTTA28040.186371237680489No Hit
CAGTGATACACTGCAGAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27570.1832473260645892No Hit
GTCCCAGACCCACTGCCACTGAACTTGTCTGGGATGCCAGTGGCCCTGCT27250.18112040751759362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27060.17985754963031497No Hit
CCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTAACTATTTAG26740.17773063108331938No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26730.17766416487872577No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26030.1730115305571729No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGTTACTGCTAACACTCTGA25740.17108401062395814No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25480.16935588930452422No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24620.16363979570947357No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC24560.1632409984819119No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24380.16204460679922686No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24380.16204460679922686No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23860.15858836416035904No Hit
GTGTTAGCAGTAACTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT23770.15799016831901652No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23750.15785723590982928No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22910.15227407472396584No Hit
GTCAGAGTGTTAGCAGTAACTATTTAGCCTGGTACCAGCAGAAACCTGGC21300.14157301578439427No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATTCTGCAGTGTA20700.13758504350877754No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACTTGTCTGGGATGCCA20450.13592338839393722No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT20010.13299887539181826No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20010.13299887539181826No Hit
CAGTAATACATAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGATA19810.13166955129994604No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19380.1288115045024207No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19160.12734924800136121No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC18960.12601992390948896No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18830.12515586324977201No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT18730.12449120120383589No Hit
CTCCAGACACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18380.12216488404305946No Hit
ATGCTAGCTCAATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA18210.12103495856496806No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17900.11897450622256607No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17650.11731285110772577No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGATATTGTGTTGACGCAGTCTC16930.11252728437698568No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16890.11226141955861123No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16850.11199555474023677No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16820.11179615612645594No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16690.11093209546673899No Hit
CTCCTGTGCAGCCTCTGAATCCACCTTTAGTACCTCTTGGATGAGTTGGATCCGCCAGGCTCCAGGGAAGGGG16560.11006803480702201No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16020.10647885975896695No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15990.1062794611451861No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15970.1061465287359989No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15820.1051495356670947No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15770.10481720464412664No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAAGTT15730.10455133982575218No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG15690.10428547500737775No Hit
GTATTACTGTGCGAGAGATGGAATAGCAGTGTATACTGGGGCTGCTTATGATATCTGGGGCCAAGGGACAATGGT15540.10328848193847356No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15500.10302261712009911No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15500.10302261712009911No Hit
GTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15270.10149389441444602No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC15200.10102863098229073No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15120.10049690134554184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAT200.002824369165.9121469
GGTATCA11100.046.362311
TCGTATG3500.040.44095640
ATCTCGT3500.040.26333237
TATCTCG3550.038.6943136
GTATCAA28100.038.0832861
CGTATGC3650.037.895741
CGAGACG4200.037.3329123
CACGAGA4150.036.95273621
ATGCCGT3600.036.61950744
ACGAGAC4350.036.0419722
CGGACTC4250.035.33544528
GACGGAC4350.034.50016426
CTTATAC4500.034.08091
ACTCCTA4250.033.8046931
CCCACGA4750.033.7165519
TCTCCGA4800.032.6500111
ATCAACG33450.032.0834583
GTATGCC4450.031.89233842
TCAACGC33950.031.7112984
Produced by FastQC (version 0.11.5)