FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983303
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGCTTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA83610.8502974159541871No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38650.3930629724510146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36460.37079109897966345No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27110.2757034200038035No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATT25530.2596351277276689No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25350.2578045627848181No Hit
GTAATATACGGCCGTGTCTTCGGCTCTCGGGCTGTTCATTTGCAGATACA23020.23410891658013858No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22980.2337021243706162No Hit
TTATTACTGCATGCAAAGTATACAGCTTCCTTTCACTTTCGGCCCTGGGA22720.2310579750087206No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22330.22709175096587725No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21060.2141760983135412No Hit
GCTGTATACTTTGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT20740.210921760637362No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20290.2063453482802351No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGGTCTAGTGCAGATATTGTGATGA20150.2049215755469067No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20040.20380289697072013No Hit
GTATATTACTGTGCGAGGTTCAGCCGGGATACAGCTGTGCTTCGGTACTT19910.20248082228977232No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19680.20014176708501855No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18620.18936177353267505No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18380.18692102027554072No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17890.18193781570889137No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17780.18081913713270475No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG17100.17390366957082407No Hit
GGATACCTGGGTCTAGTGCAGATATTGTGATGACCCAGACTCCCCTCTCTCTGTCCGTCACCCCTGGACAGCCGG16440.16719159811370451No Hit
CCCCTCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAA15780.160479526656585No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAAAGGAAG15710.1597676402899208No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15670.1593608480803984No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA15400.15661500066612224No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG15210.15468273767089086No Hit
CCAGAGAACCGATTGGAAACTTCATAGATCAGGAGTTGTGGAGGCTGGCC15150.15407254935660727No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14740.14990292920900272No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14670.14919104284233853No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14650.14898764673757733No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGCAAGACCTTTTTGT14310.14552991295663698No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13990.14227557528045778No Hit
GTGATGGCAAGACCTTTTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGATCTATGAAGTTT13790.14024161423284584No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC13650.13881784149951745No Hit
CTCCTGATCTATGAAGTTTCCAATCGGTTCTCTGGAGTGCCAGATAGGTT13210.1343431271947711No Hit
CTTTATTACTGCATGCAAAGTATACAGCTTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA13180.1340380330376293No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGG13040.13261426030430093No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGGTCTAGTGCAGATATTGTGATGACCCAGACTCCCCTCTCT12990.13210577004239793No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12880.13098709146621132No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT12800.13017350704716654No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12460.12671577326622616No Hit
GCTAATGCTCTGGATACCTGGGTCTAGTGCAGATATTGTGATGACCCAGACTCCCCTCTCTCTGTCCGTCACCC12340.125495396637659No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12320.1252920005328978No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12310.12519030248051718No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12160.1236648316948082No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGAT12110.12315634143290521No Hit
GATCAGGAGTTGTGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAAAA11970.12173256869957684No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11810.12010539986148726No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11770.11969860765196486No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11470.1166476660805469No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11460.1165459680281663No Hit
CTCCTGCATAGTGATGGCAAGACCTTTTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGAT11420.11613917581864389No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11260.11451200698055432No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11220.11410521477103193No Hit
GTCCAGGGGTGACGGACAGAGAGAGGGGAGTCTGGGTCATCACAATATCT11220.11410521477103193No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11170.11359672450912892No Hit
CCTGCATAGTGATGGCAAGACCTTTTTGTATTGGTACCTGCAGAAGCCAG11080.11268144203770353No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10980.11166446151389756No Hit
AGTCTAGTCAGAGCCTCCTGCATAGTGATGGCAAGACCTTTTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTC10960.11146106540913636No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10940.11125766930437517No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10910.11095257514723335No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10680.10861351994247959No Hit
GTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGATCTATG10550.1072914452615318No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10510.1068846530520094No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG10420.10596937058058402No Hit
GATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAAAGTATACAGCTT10290.10464729589963623No Hit
GCATAGTGATGGCAAGACCTTTTTGTATTGGTACCTGCAGAAGCCAGGCC10150.10322352316630783No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10120.10291842900916603No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10090.10261333485202424No Hit
ATGCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGAGAGAGGGGAGT10080.10251163679964365No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10070.10240993874726305No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGGTCTAGTGCAGATATTGTGATGACCCAGACTCCCCT10000.10169805238059886No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9970.10139295822345706No Hit
GTATTAGTGCTAGTGGTGGTACCACAAAATACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT9950.10118956211869587No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9930.10098616601393468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)