FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983303
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGCTTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA79150.80494008459244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47260.48062499555071014No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39570.40241919327002973No Hit
GCTGTATACTTTGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT34020.3459767741987973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28350.28831397849899776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26070.2651268225562212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24700.2511941893800792No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24590.25007551080389256No Hit
TTATTACTGCATGCAAAGTATACAGCTTCCTTTCACTTTCGGCCCTGGGA22320.22699005291349664No Hit
GTAATATACGGCCGTGTCTTCGGCTCTCGGGCTGTTCATTTGCAGATACA22110.2248543938135041No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21520.21885420872304875No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20050.20390459502310074No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATT20000.2033961047611977No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19640.19973497487549616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18740.19058215016124228No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18580.18895498132315267No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18080.18387007870412272No Hit
GTATATTACTGTGCGAGGTTCAGCCGGGATACAGCTGTGCTTCGGTACTT17940.18244630597079436No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17920.18224290986603317No Hit
GTAATAAAGTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA17660.1795987605041376No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17360.17654781893271962No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGGTCTAGTGCAGATATTGTGATGA17100.17390366957082407No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAAAGGAAG16800.17085272799940607No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16800.17085272799940607No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA16770.1705476338422643No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG16100.16373386433276418No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14790.15041141947090572No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14460.14705538374234595No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14300.14542821490425636No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA13830.14064840644236823No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13710.13942802981380104No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC13390.13617369213762187No Hit
CCAGAGAACCGATTGGAAACTTCATAGATCAGGAGTTGTGGAGGCTGGCC13300.13525840966619648No Hit
GGATACCTGGGTCTAGTGCAGATATTGTGATGACCCAGACTCCCCTCTCTCTGTCCGTCACCCCTGGACAGCCGG13150.13373293888048748No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGCAAGACCTTTTTGT13040.13261426030430093No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12690.12905482847097993No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12650.12864803626145754No Hit
CTTTATTACTGCATGCAAAGTATACAGCTTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA12580.12793614989479338No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12560.12773275379003218No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12440.12651237716146496No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12320.1252920005328978No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12310.12519030248051718No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12270.12478351027099481No Hit
CCCCTCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAA12060.12264785117100223No Hit
GATCAGGAGTTGTGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAAAA11920.12122407843767383No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG11720.11919011739006187No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11580.11776634465673348No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11290.11481710113769611No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11210.11400351671865133No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11120.11308823424722593No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGGTCTAGTGCAGATATTGTGATGACCCAGACTCCCCTCTCT11050.11237634788056174No Hit
GTATTAGTGCTAGTGGTGGTACCACAAAATACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT10960.11146106540913636No Hit
GTCCAGGGGTGACGGACAGAGAGAGGGGAGTCTGGGTCATCACAATATCT10780.10963050046628557No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT10690.10871521799486018No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10660.10841012383771838No Hit
GTCCCTGACCCGCTGCCACTGAACCTATCTGGCACTCCAGAGAACCGATT10540.1071897472091512No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10430.10607106863296462No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGAT10390.1056642764234422No Hit
CTCCTGATCTATGAAGTTTCCAATCGGTTCTCTGGAGTGCCAGATAGGTT10270.10444389979487503No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10220.10393540953297203No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGG10210.10383371148059144No Hit
GGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAAAGTATACAGCTTCCTTTCACTTTCGGCCCTGGGAC10160.10332522121868845No Hit
GTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCTATCTGGCACTCCA10110.10281673095678544No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10080.10251163679964365No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10020.10190144848536006No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10020.10190144848536006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9920.10088446796155408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTT27000.046.7711670
GGTATCA10350.039.603151
GTGGTAT2750.039.418671
CCTATAC350.001471761438.7285543
GCTTGTA754.721005E-836.133781
GTATCAA24250.035.761271
TGGTATC3150.035.4939542
TTTCGAT1251.3567496E-534.44058270
GCGATGA400.002811216333.9444925
CTTGTAT400.002837305233.880592
TCTGTCT23200.032.1992269
TAACGGC751.779179E-631.93711336
ATCAACG26800.031.6114623
AACGCAG27000.031.1278696
TCAACGC27150.030.9574684
CAACGCA27300.030.7858035
TATCAAC28050.030.4381772
AATGGTA2701.9281288E-1029.23197770
TTGCGCG704.3253072E-529.07156818
GACAGGT2450.029.0360761
Produced by FastQC (version 0.11.5)