FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367328
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58590.42849996489503617No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56810.415481874137003No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41750.30534005008308174No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41500.3035116665496501No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA38810.2838382597299258No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38410.28091284607643524No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35740.2613857099393854No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35230.25765580753118494No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35180.2572901308244986No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34680.25363336375763534No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34620.25319455170961175No Hit
ATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA33970.2484407545226895No Hit
GACTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT33600.2457347468932107No Hit
CTATAGGGGACAGTGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGT33390.24419890472512812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32730.23937197219686865No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT32240.23578834047134267No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTTCAACTTA31920.2334480095485502No Hit
ATAGGGGACAGTGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA31460.23008378384703596No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30530.22328219710267033No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30230.22108813686255238No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29850.21830899389173627No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29150.2131895199981277No Hit
CCTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACACTGTCCCCTATAGATGCTGACAGGGTGGAAGGAGA26540.19410119590910155No Hit
ACCTTGAAGGGACCCCACTTTGTAAAGTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG25420.18591003767932784No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24970.1826189473191509No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT24770.1811562404924056No Hit
CTATAATAATAAGTTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24220.17713379671885604No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23820.17420838306536543No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC23530.17208745816658474No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACACTGTCCCCTATAGA23520.17201432282524748No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23490.17179491680123568No Hit
GTGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAG23370.17091729270518852No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23050.16857696178239603No Hit
GGCCAAAAGTGTACATGAACTTATTATTATAGTGTTGGCAGTAATAAGTTGAAAAATCATCAGGCTGCAGGCTG23020.16835755575838424No Hit
CCTTCCACCCTGTCAGCATCTATAGGGGACAGTGTCACCATCACTTGCCG22900.16747993166233704No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22450.16418884130216013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21940.1604589388939596No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21840.15972758548058696No Hit
CTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTT21790.15936190877390063No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21500.15724098387511995No Hit
TTATTATAGTGTTGGCAGTAATAAGTTGAAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC21290.15570514170703736No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCA21290.15570514170703736No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20870.15263345737087222No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20850.15248718668819772No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20420.1493423670106953No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19550.14297959231435325No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTAATAGTCTGA19520.14276018629034146No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19340.14144375014627067No Hit
ATTATAGTGTTGGCAGTAATAAGTTGAAAAATCATCAGGCTGCAGGCTGC19130.1399079079781881No Hit
CACTATAATAATAAGTTCATGTACACTTTTGGCCAGGGGACCAAGCTGGA18990.13888401319946642No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18810.13756757705539563No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTA18500.1353003814739404No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18340.13413021601254418No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17860.13061971962835545No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17700.12944955416695922No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17460.12769430597486486No Hit
GTCCCCTATAGATGCTGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG17430.12747489995085304No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17400.12725549392684127No Hit
GTCTCCTTCCACCCTGTCAGCATCTATAGGGGACAGTGTCACCATCACTT17150.12542711039340962No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG17100.12506143368672332No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16580.12125839593718551No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16430.12016136581712654No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16220.11862552364904398No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16110.11782103489433407No Hit
TCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT16050.11738222284631047No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15900.1162851927262515No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15770.11533443328886706No Hit
CTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTA15660.11452994453415713No Hit
ACTTTACAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC15520.11350604975543543No Hit
TGGTGACACTGTCCCCTATAGATGCTGACAGGGTGGAAGGAGACTGGGTC15500.11335977907276089No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15400.11262842565938824No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15400.11262842565938824No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15270.1116776662220038No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15070.1102149593952585No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14930.10919106461653677No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14420.10546116220833626No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGAACTT14330.10480294413630087No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14170.10363277867490464No Hit
TTGTAAAGTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT14120.10326710196821831No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCTT14010.1024626132135084No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13980.10224320718949659No Hit
ACTTATTACTGCCAACACTATAATAATAAGTTCATGTACACTTTTGGCCA13850.10129244775211214No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13850.10129244775211214No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13790.10085363570408856No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13700.10019541763205317No Hit
GATCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA13680.10004914694937864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)