FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288663
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39710.3081488333257027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39030.30287204645434845No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29640.23000582774550057No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28000.217279459408705No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT27770.21549466384927635No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26200.20331149416100253No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25980.20160429840850558No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23990.18616193682910118No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCAGTGACCCTGCT23990.18616193682910118No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCATAATGACTGG23940.18577393779444276No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23310.1808851499577469No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22910.1777811576804797No Hit
GCATAATGACTGGCCTCTGGCGTTCGGCCAGGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT22420.17397876714082736No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21800.16916757911106317No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21480.1666843852892494No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21290.16520998895754746No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20850.16179559745255354No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20670.16039880092778327No Hit
GTCTATTACTGTGCGGGGGATTATGATCTTTGGGCACCTAAATATTACTA20510.1591572040168764No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19890.15434601598711223No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19770.15341481830393205No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18870.1464308356800808No Hit
GTTTATTACTGTCAGCAGCATAATGACTGGCCTCTGGCGTTCGGCCAGGGGACCAAGGTGGAACTCAAACGAA18840.1461980362592858No Hit
GTAATAGACAGCCGAGTCTTCGACTCTCAGGTTGTTCATTTGGAGATACA18710.14518923876917394No Hit
CTCCTGCAGGGCCAGTCAGAGTATTGGCAGCAACTTAGCCTGGTACCAGCAAATCCCTGGCCAGGCTCCCAGGCT18300.14200764668497504No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17970.13944685305622959No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17310.13432526579873869No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17200.1334716679224902No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16850.13075567467988142No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCC16840.13067807487294972No Hit
GTCATTATGCTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT16170.12547888780852715No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15820.12276289456591832No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15710.12190929668966984No Hit
GTCTGTGTCTCCAGGAGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGGCAGCAACTTAGCCTGGTA15470.1200469013233095No Hit
GAGTATTGGCAGCAACTTAGCCTGGTACCAGCAAATCCCTGGCCAGGCTCCCAGGCTCCTCATCTATCGTGCATC15450.11989170170944616No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15000.11639971039752053No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGAGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGGCAG14790.11477011445195524No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14670.11383891676877507No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCA14650.11368371715491173No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCCTGGCCGAACGCC14490.11244212024400482No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14390.11166612217468803No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13970.10840693028355745No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGT13950.1082517306696941No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13940.10817413086276241No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13900.10786373163503568No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13760.10677733433799216No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13740.1066221347241288No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13720.10646693511026543No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13720.10646693511026543No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACCGCCA13520.10491493897163183No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13520.10491493897163183No Hit
GAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTC13280.1030525436052715No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGG13080.10150054746663789No Hit
CATCTATCGTGCATCCAGCAGGGTCACTGGTCTCCCAGCCAGGTTCAGTGGCGGTGGGTCTGGGACAGAGTTCA13000.10087974901118446No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12980.1007245493973211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCA200.005787742354.99019265
TCCGAAC200.007263902651.9208743
GTCGTAT200.00747946451.53827739
CGCGTAT200.00747946451.53827739
AATCGCG200.007617913751.29973636
GGTATCA17450.045.9712071
AATGTCG306.589431E-445.59976236
CGTATGC4950.038.3290541
TCTGGAG8900.037.78030470
TCGTATG4800.037.2823240
ATGCCGT5050.037.0865144
GTATCAA40450.036.985951
GGCAATC4600.035.59323533
CGAGACA5450.035.41303623
GTATGCC5400.035.19762442
CACGAGA5500.035.08564821
ATCTCGT4300.035.03777337
ATCGTAT601.38088635E-534.3588539
AATATCG501.9596107E-434.19982536
CCCACGA5750.034.1396819
Produced by FastQC (version 0.11.5)