FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288663
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50170.38931823137624033No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40580.3149000165287589No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33030.2563121622953402No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29160.2262810370127799No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28640.22224584705233252No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT27510.21347706886905268No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCATAATGACTGG24220.18794673238852982No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23820.18484274011126262No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22570.17514276424480257No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22480.17444436598241742No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22040.17102997447742352No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCAGTGACCCTGCT21510.16691718471004444No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20880.16202839687334858No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20770.1611747989971001No Hit
GCATAATGACTGGCCTCTGGCGTTCGGCCAGGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT20380.15814840652676457No Hit
GTCTATTACTGTGCGGGGGATTATGATCTTTGGGCACCTAAATATTACTA20150.15636361096733592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19630.15232842100688854No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19200.14899162930882628No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18770.14565483761076403No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18650.14472363992758386No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18540.14387004205133538No Hit
GTTTATTACTGTCAGCAGCATAATGACTGGCCTCTGGCGTTCGGCCAGGGGACCAAGGTGGAACTCAAACGAA18330.14224044610577008No Hit
CTCCTGCAGGGCCAGTCAGAGTATTGGCAGCAACTTAGCCTGGTACCAGCAAATCCCTGGCCAGGCTCCCAGGCT17930.13913645382850287No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17770.13789485691759598No Hit
GTAATAGACAGCCGAGTCTTCGACTCTCAGGTTGTTCATTTGGAGATACA17650.13696365923441584No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17560.1362652609720307No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCC17290.13417006618487531No Hit
GTCATTATGCTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT16660.12928127834817948No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16380.12710848375409242No Hit
GAGTATTGGCAGCAACTTAGCCTGGTACCAGCAAATCCCTGGCCAGGCTCCCAGGCTCCTCATCTATCGTGCATC16310.12656528510557066No Hit
GTCTGTGTCTCCAGGAGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGGCAGCAACTTAGCCTGGTA15910.12346129282830344No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15810.12268529475898665No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15630.1212884982342164No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCCTGGCCGAACGCC14890.11554611252127203No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14590.11321811831332164No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14540.11283011927866324No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGT14540.11283011927866324No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCA14530.11275251947173155No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGAGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGGCAG14350.1113557229469613No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACCGCCA14210.11026932564991779No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14080.10926052815980593No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14060.10910532854594258No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13810.10716533337265058No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13500.10475973935776847No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13440.1042941405161784No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13430.10421654070924673No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13300.10320774321913487No Hit
GTTCAGTGGCGGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGTC13160.10212134592209135No Hit
GAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTC12920.10025895055573102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)