FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057110
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATGAGGCGT43170.4083775576808469No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42860.4054450341024113No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40510.38321461342717406No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30450.2880494934302012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28040.2652514875462345No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26730.2528592104889747No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA26370.24945369923659788No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26040.24633198058858588No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25130.2377236049228557No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24460.2313855700920434No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24200.22892603418754906No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23780.22495293772644287No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC23700.2241961574481369No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT23370.22107443880012484No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG23170.21918248810435997No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22920.2168175497346539No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA22540.21322284341270067No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21930.2074523937906178No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAGCACTATAAAACTCATTTTTCATTCA21710.20537124802527648No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21520.20357389486429983No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21080.19941160333361713No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGG20910.19780344524221702No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT19680.18616794846326304No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA19610.18550576571974534No Hit
CTATAAAACTCATTTTTCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT19600.1854111681849571No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC19070.1803974988411802No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATGAGGCGTCTA18740.17727578019316817No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT18700.1768973900540152No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGAAAAATGAGTTTTATAGTG18190.1720729157798148No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18080.17103234289714409No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17610.16658625876209665No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCACC17440.16497810067069651No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17310.16374833271844935No Hit
ACTTTAGAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGC17150.16223477216183746No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16770.15864006583988421No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16560.1566535176093311No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16230.15353179896131908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16100.15230203100907191No Hit
ACTTATTACTGCCAGCACTATAAAACTCATTTTTCATTCACTTTCGGCCC15530.14690997152614205No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15310.1448288257608007No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15210.14388285041291826No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15200.14378825287813No Hit
GTTTTATAGTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC15120.14303147259982404No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15110.14293687506503583No Hit
TTCTAAAGTAGACGCCTCATAGATCAGGAACTTAGGGGCTTTCCCTGGTT14630.13839619339520012No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG14370.13593665749070577No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13830.13082839061214066No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13730.12988241526425823No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13480.12751747689455212No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13420.12694989168582266No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13400.12676069661624617No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCC13260.12543633112921077No Hit
TCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAGCACTAT12760.1207064543897986No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTG12660.11976047904191617No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12550.11871990615924549No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12510.11834151602009252No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCA12500.11824691848530428No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12470.11796312588093953No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12450.11777393081136306No Hit
ACCTTGATGGGACCCCGCTTTCTAAAGTAGACGCCTCATAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTG12290.11626037025475117No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12210.11550358997644522No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATGAGGCGTCTACTTTAGAAAGCGGGGTCCCAT12200.11540899244165696No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12030.11380083435025683No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11910.11266566393279792No Hit
CCTCATAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA11620.10992233542393885No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11610.1098277378891506No Hit
GCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGA11490.1086925674716917No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11380.10765199458902101No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11300.10689521431071507No Hit
GATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC11300.10689521431071507No Hit
TCTATGAGGCGTCTACTTTAGAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11290.10680061677592681No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11250.10642222663677385No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAA11230.10623303156719736No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11190.10585464142804438No Hit
GCCCCTAAGTTCCTGATCTATGAGGCGTCTACTTTAGAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT11120.10519245868452669No Hit
GTTCCTGATCTATGAGGCGTCTACTTTAGAAAGCGGGGTCCCATCAAGGT11100.1050032636149502No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11080.10481406854537371No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11060.10462487347579721No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11050.10453027594100898No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10820.10235453264087938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)