FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057110
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69840.660669182961092No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44260.41868868897276534No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATGAGGCGT39040.36930877581330235No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32950.3116988771272621No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31890.30167153843970823No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29470.27877893502095336No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA24000.2270340834917842No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23510.22239880428716027No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23510.22239880428716027No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23230.2197500733130895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22220.21019572229947686No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC21770.2059388332340059No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21630.2046144677469705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20990.19856022552052294No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20970.19837103045094645No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20850.1972358600334875No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAGCACTATAAAACTCATTTTTCATTCA20190.19099242273746347No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA19740.18673553367199253No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG19310.18266783967609804No Hit
CTATAAAACTCATTTTTCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT19050.1802083037716037No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA18960.17935692595850952No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGAAAAATGAGTTTTATAGTG18950.17926232842372128No Hit
GTTTTATAGTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC18810.17793796293668587No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18520.17519463442782682No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18310.17320808619727368No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC18290.17301889112769722No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGG18100.1712215379667206No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18100.1712215379667206No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17860.16895119713180273No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCG17520.16573488094900246No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17490.16545108834463773No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATGAGGCGTCTA17490.16545108834463773No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA17460.165167295740273No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT17330.16393752778802584No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16990.16072121160522557No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16550.15655892007454286No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT16530.15636972500496638No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16430.15542374965708394No Hit
ACTTTAGAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGC15990.1512614581264012No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCACC15430.14596399617825961No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14880.1407611317649062No Hit
ACTTATTACTGCCAGCACTATAAAACTCATTTTTCATTCACTTTCGGCCC14660.13867998599956485No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14660.13867998599956485No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14580.1379232057212589No Hit
TTCTAAAGTAGACGCCTCATAGATCAGGAACTTAGGGGCTTTCCCTGGTT14560.13773401065168242No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14310.13536907228197634No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13700.12959862265989347No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13450.1272336842901874No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13380.12657150154666968No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13250.12534173359442252No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13170.12458495331611659No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12990.1228821976899282No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12780.12089564945937509No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG12340.11673335792869238No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12270.11607117518517467No Hit
ACCTTGATGGGACCCCGCTTTCTAAAGTAGACGCCTCATAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTG12270.11607117518517467No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12070.1141792244894098No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCC12060.11408462695462157No Hit
TCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAGCACTAT11920.11276026146758615No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11880.11238187132843318No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11830.11190888365449196No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG11760.11124670091097427No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11560.10935475021520938No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCA11510.10888176254126816No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGGGAGCCAGAGCAGCAGGAGTCCCAGGAG11450.10831417733253872No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11190.10585464142804438No Hit
CCTCATAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA11100.1050032636149502No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11000.10405728826706777No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAA10870.10282752031482059No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10740.10159775236257343No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10710.1013139597582087No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTG10600.10027338687553801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA150.002113145570.914954
TACGTAT450.00958771848.39513470
GTGGTAT3550.042.7854231
GTATCAA27700.035.2151641
ATCCCGA702.8887653E-530.84281260
ATCAACG30500.030.764983
TCAACGC30700.030.567454
GTACGTA600.00490542630.29551369
TATCAAC31600.030.2281112
CAACGCA31350.030.0399285
AATGGTA5150.029.60090670
GACAGGT1600.029.5338251
AACGCAG32100.029.4432146
ACGCAGA32450.029.0216267
CGCAGAG32550.028.9324658
AGAGTAC32800.028.80940211
ACTTCCA5350.028.49433370
GCAGAGT33800.027.9623439
CTTGTAT757.1536124E-527.0023562
GAGTCGG500.00860638527.0023541
Produced by FastQC (version 0.11.5)