FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859252
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22470.26150651962404514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21530.25056677202962574No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20660.24044168649011No Hit
GTATATTACTGTGCGAGAGATTACTATGACAGTAGTGGTTATCTACCGGG18620.21670010660434888No Hit
CTATAATAACTGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17780.20692416194550609No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17470.20331637284521886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17420.20273447137743061No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15740.18318258205974497No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15340.1785273703174389No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15240.17736356738186237No Hit
CATCTATGCTGCATCCACTAGGGCCACTGGTCTCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCA14420.16782038331013485No Hit
GTTATTATAGTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATT14400.16758762272301955No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14330.16677296066811598No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA14220.1654927774389818No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14220.1654927774389818No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14160.1647944956776359No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14110.16421259420984763No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGC14020.16316517156782878No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13840.161070326283791No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13200.15362198749610126No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAACAG12420.14454432459860436No Hit
CTCCTGCAGGGCCAGTCAGAATATTAACAGCAACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT12140.1412856763789901No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12080.1405873946176442No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGACCCACTGCCA11590.1348847602333192No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11420.13290629524283912No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11410.13278991494928147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11320.1317424923072626No Hit
GTTTATTACTGTCAGCACTATAATAACTGGCCTCCGTGGACGTTCGGCCA11280.131276971133032No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11130.1295312667296672No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11000.12801832291341772No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10500.12219930823553508No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAACAGCAACTTAGCCTGGTA10460.12173378706130447No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10290.11975532207082439No Hit
ATAATAACTGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT10260.11940618119015142No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCACTGAACCTGACTGGGAGACCA9890.1151001103285183No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9700.1128888847509229No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9570.11137594093467343No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9530.11091041976044282No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9480.11032851829265455No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9430.10974661682486629No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9290.10811729271505914No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9240.10753539124727088No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9230.10741901095371323No Hit
GTCCCAGACCCACTGCCACTGAACCTGACTGGGAGACCAGTGGCCCTAGT9180.10683710948592497No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGCCAGTTATTATAGTG9170.1067207291923673No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9050.10532416566967548No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9030.10509140508256018No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9010.10485864449544487No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCACTATAATAACTGG8940.1040439824405413No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTTGCTGTTA8890.10346208097275304No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8870.10322932038563774No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8600.10008705245958113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATCG450.00761488951.29771870
TAGGCGA200.007769332351.04345737
AAGCGTC200.00827335150.2382626
GACAGGT1100.039.4479831
GATTGTG501.0986184E-437.73380765
GGTATCA5350.036.8105551
AAAACCG1301.0989475E-535.51380570
TCTGGAG5900.035.2128470
CGGGATT400.003032266633.42382417
GCTCTAA400.003047895933.3887834
AGCGCGG400.003049636333.3848910
GAGTTTA3000.032.2664261
GTATCAA10200.031.0883431
GCCCGTT450.00542606329.6771897
TGTTAGA12900.028.63128570
ACGGGGC954.2296415E-728.14309916
CCCGTTC606.5674563E-427.8223638
CCGTTCC606.5674563E-427.8223639
TGTAAAC500.007761001627.5800841
GCGTTGT5050.027.17784562
Produced by FastQC (version 0.11.5)