FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277174
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51990.40707061058242655No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47660.37316763416731No Hit
GAGTTACAGTTCCCCTGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT46510.36416337946121674No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35410.277252747080664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34960.2737293430652362No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34530.27036253478382744No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33820.2648033862261524No Hit
GATCTATGGTGCATCCCGTTTGCACACCGGGGCCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA32730.25626891872211616No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30300.23724253703880596No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCTTCACTTGCCGGGCAAGTCAGAACATTACCAA29630.231996579949169No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28950.22667232499252254No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27750.21727658095138172No Hit
CTGTAACTCTGTTGACAGTAGTGAATTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC27630.21633700654726762No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27380.21437955987202995No Hit
GTAGGAGACAGAGTCACCTTCACTTGCCGGGCAAGTCAGAACATTACCAACTTTTTAAATTGGTATCAGCAGACA27350.21414466627100145No Hit
CCATAGATCAGGAGCTTTGGGGCTTTCCCTGGTGTCTGCTGATACCAATT27020.21156083665968772No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26830.2100731771865071No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26290.2058450923679937No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25900.20279147555462293No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25430.1991114758051761No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23690.18548764694552192No Hit
AGTCAGAACATTACCAACTTTTTAAATTGGTATCAGCAGACACCAGGGAA23200.18165105146205607No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACTCAGTC23080.18071147705794197No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGACAGGGGAA23080.18071147705794197No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAATTCACTACTGTCAACAG22670.1775012645105522No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22510.1762484986384001No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22280.17444764769718143No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT21890.17139403088381067No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21250.16638296739520222No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20970.16419062711893603No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAATTCACTACTGTCAACAGAGTTACAGTTCC20350.15933615936434659No Hit
CACCAGGGAAAGCCCCAAAGCTCCTGATCTATGGTGCATCCCGTTTGCAC20250.15855318069425153No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20000.15659573401901386No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19930.1560476489499473No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19230.15056679825928182No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAATTCACTACTGTCAACAGAGTTACAGTTCCCCTGTCACTTT18890.1479046707809586No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG18320.14344169236141668No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18300.14328509662739766No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18140.14203233075524557No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACTCAGTCTCCATCCTCC18070.141484245686179No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17920.14030977768103642No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17790.13929190540991282No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17670.13835233100579875No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT16710.13083573577288607No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16610.130052757102791No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGGCCCCG16600.1299744592357815No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCTTCACTTGCCGGGCAAGTCAGA16590.129896161368772No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16100.12605956588530615No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15830.12394552347604947No Hit
ATTCACTACTGTCAACAGAGTTACAGTTCCCCTGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15450.12097020452968821No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15310.1198740343915551No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15180.11885616212043151No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15120.11838637491837448No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15110.11830807705136497No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15110.11830807705136497No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15080.11807318345033643No Hit
GTAGTGAATTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14670.11486297090294666No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14390.11267063062668048No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14350.11235743915864244No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14200.11118297115349984No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14140.11071318395144279No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13890.10875573727620513No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13870.10859914154218611No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13870.10859914154218611No Hit
GTGCAAACGGGATGCACCATAGATCAGGAGCTTTGGGGCTTTCCCTGGTG13820.10820765220713857No Hit
GTCTGCAACCTGAAGATTTTGCAATTCACTACTGTCAACAGAGTTACAGTTCCCCTGTCACTTTCGGCGGAGGGA13680.10711148206900548No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13580.10632850339891041No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13540.10601531193087238No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13460.10538892899479634No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAATTCA13400.10491914179273928No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13150.10296169511750161No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13140.1028833972504921No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13080.10241361004843506No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12930.10123914204329247No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12820.10037786550618788No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTG12800.10022126977216887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAC200.00712499852.17497645
GGTTGCG200.00801562850.64354723
CAATACG200.00803302650.6157761
GTGGTAT2550.039.6986471
GTATCAA27400.035.591141
ATGCCGT3950.035.1455744
GACGTAG5400.034.41051526
ATGTCGT601.4306499E-534.1816337
AGACGTA5550.034.07454325
GAATTAG400.002770983834.04520834
ACGTAGA5450.033.49067727
CGAGACG5650.033.4635823
CGTATGC4150.033.2389141
CACGAGA5800.033.17115421
TCGTATG4150.033.15794840
CTTATAC5950.032.8931661
ACGAGAC5850.032.89279622
CGTAGAG5600.032.612828
GAGACGT5850.032.3233324
ATCTCGT3450.031.704737
Produced by FastQC (version 0.11.5)