FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277174
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58250.4560850753303779No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51720.4049565681731699No Hit
GAGTTACAGTTCCCCTGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT44800.350774444202591No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39560.3097463618896094No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36960.2893889164671376No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35180.2754518961394454No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32880.25744338672725875No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31020.24287998346349046No Hit
GATCTATGGTGCATCCCGTTTGCACACCGGGGCCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA30320.237399132772825No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCTTCACTTGCCGGGCAAGTCAGAACATTACCAA29730.2327795586192641No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27730.21711998521736273No Hit
GTAGGAGACAGAGTCACCTTCACTTGCCGGGCAAGTCAGAACATTACCAACTTTTTAAATTGGTATCAGCAGACA27030.21163913452669722No Hit
CTGTAACTCTGTTGACAGTAGTGAATTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC26080.20420083716079407No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25800.20200849688452788No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25620.20059913527835674No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25510.19973785874125216No Hit
CCATAGATCAGGAGCTTTGGGGCTTTCCCTGGTGTCTGCTGATACCAATT25040.19605785899180533No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24470.19159488057226345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24260.1899506253650638No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24000.18791488082281663No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGACAGGGGAA22970.17985020052083742No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22650.17734466877653318No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22550.1765616901064381No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22510.1762484986384001No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT22180.17366466902708635No Hit
AGTCAGAACATTACCAACTTTTTAAATTGGTATCAGCAGACACCAGGGAA21950.1718638180858677No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACTCAGTC21520.1684970098044589No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21520.1684970098044589No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAATTCACTACTGTCAACAGAGTTACAGTTCC20940.16395573351790751No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20570.16105871243855574No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAATTCACTACTGTCAACAG20320.15910126576331807No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19710.15432509587573814No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19620.1536204150726526No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19340.1514280747963864No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19190.15025360679124378No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18680.14626041557375893No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAATTCACTACTGTCAACAGAGTTACAGTTCCCCTGTCACTTT18620.1457906283717019No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG18610.1457123305046924No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17950.14054467128206494No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17940.14046637341505544No Hit
CACCAGGGAAAGCCCCAAAGCTCCTGATCTATGGTGCATCCCGTTTGCAC17380.13608169286252306No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17190.13459403338934242No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACTCAGTCTCCATCCTCC17100.13388935258625684No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16920.13247999098008573No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGGCCCCG16460.1288782890976484No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16390.12833020402858186No Hit
ATTCACTACTGTCAACAGAGTTACAGTTCCCCTGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16360.12809531042755334No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16330.12786041682652483No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCTTCACTTGCCGGGCAAGTCAGA16240.12715573602343924No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16150.1264510552203537No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16030.1255114808162396No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15960.12496339574717306No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15910.12457190641212551No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14810.11595914104107975No Hit
GTAGTGAATTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14660.11478467303593716No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14490.11345360929677555No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14460.11321871569574703No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14460.11321871569574703No Hit
GTGCAAACGGGATGCACCATAGATCAGGAGCTTTGGGGCTTTCCCTGGTG14070.11016509888237624No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAATTCA13900.10883403514321463No Hit
GTCTGCAACCTGAAGATTTTGCAATTCACTACTGTCAACAGAGTTACAGTTCCCCTGTCACTTTCGGCGGAGGGA13620.10664169486694842No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13540.10601531193087238No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13520.10585871619685336No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13460.10538892899479634No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13340.10444935459068225No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13300.10413616312264422No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG13260.10382297165460619No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13200.10335318445254915No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13160.10303999298451112No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12920.10116084417628295No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12910.10108254630927345No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12870.10076935484123542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCG150.002152056270.5903151
TTCCCGA400.00627190753.8814270
GTGGTAT3550.048.3325771
AATTCGC559.716314E-846.28269661
GAATTCG755.4387783E-1043.44119660
GTACGTA500.001995995636.3988869
GTATCAA29000.033.7592771
TATCAAC33450.029.1721252
ATCAACG33900.028.7848833
CAACGCA34550.028.4381265
TCAACGC34450.028.4230024
AACGCAG34700.028.3163036
GACAGGT2400.028.0360531
TAACGTG500.00819598827.274635
ATTCGCA956.7721667E-626.90115762
ACGCAGA36400.026.808957
CGCAGAG36550.026.6989298
AGAGTAC37400.026.08702311
GCAGAGT37800.025.8160279
GTACGGA951.2771026E-524.7897761
Produced by FastQC (version 0.11.5)