FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601820
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG28000.4652553919776678No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGGTGTGGCTTTCGG26670.44315576085872854No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16960.28181183742647303No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGCCAC15730.26137383270745407No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15220.2528995380678608No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14410.23944036422850687No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC14050.23345850918879402No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13310.2211624738293842No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT12980.2156791067096474No Hit
GTATTAGTTTCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT12510.20786946263002226No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA11640.19341331295071618No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11570.19225017447077197No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG11430.18992389751088365No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10870.1806187896713303No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10150.16865507959190454No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10150.16865507959190454No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT9770.16234089927220766No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9420.15652520687248678No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9380.1558605563125187No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9090.15104183975274998No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8830.14672161111295737No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8830.14672161111295737No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGCCACACCATTCAGG8740.14522614735302913No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8730.1450599847130371No Hit
CCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCTC8440.14024126815326843No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8390.1394104549533083No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8330.13841347911335616No Hit
AACTAATACTGTACACTGGTACCAGCAGCTCCCAGGAACGGCCCCCCAAC7830.13010534711375496No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7790.12944069655378684No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7670.12744674487388258No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG7670.12744674487388258No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7610.1264497690339304No Hit
CTCATATACAGTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCC7580.12595128111395432No Hit
CCCCTGAGGGCCGCCGATTATTACTATAGATGAGGAGTTGGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTGTA7570.12578511847396232No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7500.12462197999401814No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC7500.12462197999401814No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7440.12362500415406599No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7380.12262802831411385No Hit
GTGTATTACTGTGCGAGAGGAGGTGTCGGAGGGAATTACTACGACTGGGGCCAGGGAACCCTGGTCACCGTCTC7370.12246186567412183No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT7340.12196337775414576No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7180.11930477551427338No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6960.11564919743444883No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT6950.1154830347944568No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT6760.11232594463460835No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA6760.11232594463460835No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6720.11166129407464026No Hit
ATCATATACTATGCAGACTCTGTGAGGGGCCGATTCACCGTCTCCAGAGACAATGCCAAGAACTCGCTGTATCTG6650.11049815559469608No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6360.1056794390349274No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGAAACTAATACTGTACACTGGTACCAGCAGCTCCCAGGAACGGCCCC6340.10534711375494335No Hit
ACTGTACACTGGTACCAGCAGCTCCCAGGAACGGCCCCCCAACTCCTCAT6170.10252234887507893No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGCCACAC6060.10069455983516666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA309.243397E-657.37043838
ATATCGT309.284753E-657.32724437
CCGTCGT252.4676215E-455.6392847
TCGTATG2600.051.7851440
CGCGCTG200.007462619351.5643236
CGTATGC2900.051.25015641
TTCTACG200.007651885551.23872411
AGGTACG200.007651885551.2387242
GGTACGG200.007651885551.2387243
ATGCCGT2800.049.49790644
CATATCG352.3203613E-549.10887536
TCTGGAG4750.048.44268870
GTATGCC3000.048.43039742
CTAGCGC3550.048.2156328
CTTGAAA3250.047.73753457
ACTAGCG3650.046.85173827
CACGAGA3800.046.75186521
GCGCTCA3600.046.65720731
TGCTTGA3000.045.70560555
GACTAGC3750.045.59477626
Produced by FastQC (version 0.11.5)