FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816558
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCTCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT74370.40940063570775065No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGCTGATGGTGAGTGTGAATTCTGTT67860.3735636296776651No Hit
GTAATAGACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAGATATA62720.3452683591715761No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49620.27315395379613533No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49300.2713923805350558No Hit
GTCTATTACTGTGCGAGAGAGCAGTATTACTATGACAGTAGTGGTTATCT45180.24871212479865767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38150.21001256221931808No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33100.18221273419290768No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31510.17345991705191907No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29920.16470709991093044No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29700.1634960182939383No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29570.16278037915662477No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA28640.15766080686661257No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27790.15298162789187023No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26280.14466920406615147No Hit
TAGTAATACTGCTCTCTCGCACAGTAATAGACAGCCGTGTCCCCGGCTCT26110.14373336827120303No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG26020.14323792579152442No Hit
GACTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT25980.14301772913388947No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25570.14076071339313143No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGCTAC25290.13921933678968687No Hit
GAATTCACACTCACCATCAGCGGCCTGCAGCCTGATGATTTTGCAACTTA24710.1360264852539803No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24570.13525579695225806No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24140.13288868288268252No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23520.12947563468934103No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT23270.12809940557912272No Hit
ATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA23250.12798930725030525No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23210.12776911059267032No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23100.12716356978417423No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22870.1258974390027734No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22800.12551209485191223No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22470.12369547242642404No Hit
GTGTGAATTCTGTTCCAGATCCACTGCCTCTGAACCTTGATGGGACCCCA22160.12198894832975331No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21520.11846580180759436No Hit
GTATTACTATGACAGTAGTGGTTATCTTGACTACTGGGGCCAGGGAACCC21430.11797035932791576No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20970.11543809776511402No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20960.11538304860070529No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20530.11301593453112975No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20390.11224524622940749No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19510.10740091976143894No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18840.1037126257460538No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC18560.10217124914260926No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18480.1017308558273394No Hit
CAGTATAATAGTTATTCTCCGTGGACGTTCGGCCCAGGGACCAAGGTGGA18290.10068492170357346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG19450.050.43051570
ATCTCGT3450.047.73107537
CTAACGC652.5465852E-1046.9328163
CGCTAAT4000.046.05232232
AATCTCG3700.045.3624436
ACTCGCT4100.044.80744629
TCGCTAA4200.043.8217231
TCGTATG3800.043.5727240
CGTATGC4000.043.21325341
ATGCCGT3950.043.1308644
GCTAATC4200.042.27709633
ACACTCG4400.041.6913527
GTATCAA48800.040.575141
CACTCGC4550.040.34696228
GTATGCC4400.040.1606742
CGAGACA4750.038.55766323
CACGAGA4750.038.54598621
ACGAGAC4900.037.3690922
CTCGCTA4950.037.1460830
CTAATCT4550.036.80623234
Produced by FastQC (version 0.11.5)