FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816558
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCTCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT71930.39596863959201967No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGCTGATGGTGAGTGTGAATTCTGTT66510.36613199248248607No Hit
GTAATAGACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAGATATA56660.31190856553988366No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49500.2724933638232305No Hit
GTCTATTACTGTGCGAGAGAGCAGTATTACTATGACAGTAGTGGTTATCT45670.2514095338546856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43820.24122543843906993No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39770.2189305268535329No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39390.216838658606001No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35900.19762650022735304No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA30320.1669090664872798No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28200.1552386436326283No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26980.14852264557476283No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26940.14830244891712788No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25650.14120110670840127No Hit
GACTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT25400.13982487759818293No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25350.13954963177613927No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25270.13910923846086942No Hit
GTGTGAATTCTGTTCCAGATCCACTGCCTCTGAACCTTGATGGGACCCCA25120.1382835009947384No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24800.13652192773365893No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24550.1351456986234406No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24410.1343750103217183No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGCTAC24320.13387956784203972No Hit
TAGTAATACTGCTCTCTCGCACAGTAATAGACAGCCGTGTCCCCGGCTCT24220.1333290761979524No Hit
GAATTCACACTCACCATCAGCGGCCTGCAGCCTGATGATTTTGCAACTTA23530.1295306838537498No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23280.12815445474353143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23190.12765901226385284No Hit
ATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA22520.12397071824846771No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG22200.12220914498738823No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22100.12165865334330091No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21780.11989708008222143No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21520.11846580180759436No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20060.11042862380391928No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19970.10993318132424068No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19360.10657518229530794No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19100.10514390402068087No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18850.10376767491046253No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18840.1037126257460538No Hit
TAGTTATTCTCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT18520.10195105248497432No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18520.10195105248497432No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA18480.1017308558273394No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18230.10035462671712106No Hit
GTATTACTATGACAGTAGTGGTTATCTTGACTACTGGGGCCAGGGAACCC18230.10035462671712106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG18950.052.26109370
GTATCAA54400.037.2595671
CTCTGGA21800.035.00055369
GCGTGTT18050.033.12812451
CGTGTTC17900.033.08660552
TAGCGTG18300.032.73002249
AGTGTCT19450.032.49873764
GTGTCTC19500.032.20947365
AAGTGTC19350.032.19317263
ATCAACG63450.031.7318363
GAAGTGT19750.031.43182862
AACGCAG64100.031.4100636
TCAACGC64450.031.3462244
ATAGCGT19000.031.28275348
CAACGCA65100.030.9795555
TATCAAC65650.030.56372
GTGTTCT20000.030.00895553
AGAGTAC67500.029.77123811
ACGCAGA67700.029.7398097
CGCAGAG67950.029.6303948
Produced by FastQC (version 0.11.5)