FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271310
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT49770.3914859475658966No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49160.3866877472842973No Hit
GCCTTGGGCTGACCTAGGACGGCCAGCTGTGTCCCTCCGCCAAATTTCACCCCTGAAGGATCGGTACCAGTCTCC39860.31353485774516054No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37660.2962298731229991No Hit
ATATTACCTGTGGGACCAACAACATTGGGACTGGCAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCT35890.2823072264042602No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35210.2769584129755921No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTGTGTCCCTC31690.2492704375801339No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31690.2492704375801339No Hit
CAGTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATC27300.2147391273568209No Hit
CCCCAGGACAGACGGCCAATATTACCTGTGGGACCAACAACATTGGGACT24770.19483839504133532No Hit
GACCTAGGACGGCCAGCTGTGTCCCTCCGCCAAATTTCACCCCTGAAGGA24030.1890176274866083No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23810.18728712902439218No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23610.18571394860419568No Hit
CGGCCACCCTGACCATCACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGATGTGGGAGACTGGTA22440.1765108431460462No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21780.17131934775939778No Hit
GTACCAGTCTCCCACATCTGACAGTAATAGTCGGCCTCATCCCCGGCCTC21670.17045409852828972No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21550.16951019027617184No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21180.16659980649880832No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21170.1665211474777985No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21160.16644248845678866No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20730.16306015055336623No Hit
CTGATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG20700.16282417349033673No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20590.16195892425922867No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGACCTCTGATGTGCTGACT20510.16132965209115008No Hit
GGGTGGCCGTGTCCCCAGAGTTGGAGCCAGAGAAGCGCTCAGGGATCCCT20330.15991378971297324No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGA20060.157789996145708No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC20010.15739670104065884No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20010.15739670104065884No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19920.15668876985157043No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19650.15456497628430516No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG19460.1530704548851185No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19410.15267715978006938No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19150.15063202523381394No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATCCA19010.14953079893967638No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18930.14890152677159782No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT18890.1485868906875585No Hit
ACATTGGGACTGGCAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCC18430.14496857572110658No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18240.1434740543219199No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18150.1427661231328315No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18080.1422155099857627No Hit
GACTGGTACCGATCCTTCAGGGGTGAAATTTGGCGGAGGGACACAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGC17770.13977708033445815No Hit
CTATTACTGTCAGATGTGGGAGACTGGTACCGATCCTTCAGGGGTGAAATTTGGCGGAGGGACACAGCTGGCCGT17370.13663071949406516No Hit
ATATTACTGTGTGTACTCGGGGACTTATTATTCATACTTTGACTACTGGGGCCAGGGAACCCGGGTCACCGTCTC17280.13592278830497675No Hit
GTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGTGATGGTCAGGGTGG16650.13096726998135783No Hit
GACCATCACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGA16620.13073129291832833No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16470.12955140760318098No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16380.12884347641409255No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGACCTCTGA16310.12829286326702377No Hit
GTATATTACTGTGTGTACTCGGGGACTTATTATTCATACTTTGACTACTGGGGCCAGGGAACCCGGGTCACCGT16240.127742250119955No Hit
CTCTTTGACCTCTGATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAATATTAC15950.1254611385106701No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15920.12522516144764062No Hit
CCTCTCTCACTGCACAGGCTCTTTGACCTCTGATGTGCTGACTCAGCCAC15880.12491052536360131No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGACCTCTGATGTGCTGACTCAGC15800.12428125319552273No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTGTGTC15230.11979768899796273No Hit
CACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGATGTGGG15090.11869646270382518No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTGTGTCCCTCCGCCAAATTTCACCC15040.11830316759877607No Hit
ACCCTGGTGATGGTCAGGGTGGCCGTGTCCCCAGAGTTGGAGCCAGAGAA14720.11578607892646169No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAATATTACCTGTGGGACCAACAACATTGGGACTGGCAGTGTG14680.11547144284242239No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC14320.11263971808606871No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14300.11248240004404905No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14210.11177446885496063No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14190.11161715081294099No Hit
GAGTTGGAGCCAGAGAAGCGCTCAGGGATCCCTGAGGGCCGATTTTTATTTTCATATATGACCAACAGAGGGGC13980.10996531137173467No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13790.108470789972548No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13560.10666163248932205No Hit
GGCTCTTTGACCTCTGATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAATATTAC13540.10650431444730239No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13480.10603236032124344No Hit
GACTATTACTGTCAGATGTGGGAGACTGGTACCGATCCTTCAGGGGTGAA13480.10603236032124344No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13440.10571772423720413No Hit
CTCACTGCACAGGCTCTTTGACCTCTGATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG13390.10532442913215503No Hit
CAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGTGATGGTCAGGGT13330.10485247500609607No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13220.103987225774988No Hit
GGCCAGGCCCCTCTGTTGGTCATATATGAAAATAAAAATCGGCCCTCAGG13040.10257136339681117No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12940.1017847731867129No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12890.1013914780816638No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12850.1010768419976245No Hit
CAGGGATCCCTGAGCGCTTCTCTGGCTCCAACTCTGGGGACACGGCCACC12720.10005427472449678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)