FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271310
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97110.7638577530264058No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43040.3385484264262847No Hit
GCCTTGGGCTGACCTAGGACGGCCAGCTGTGTCCCTCCGCCAAATTTCACCCCTGAAGGATCGGTACCAGTCTCC41040.3228166222243198No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39390.3098378837576988No Hit
ATATTACCTGTGGGACCAACAACATTGGGACTGGCAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCT35910.28246454444627983No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35570.2797901377319458No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTGTGTCCCTC30150.2371569483446209No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29130.2291337282016188No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26390.2075811564449269No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26270.206637248192809No Hit
CAGTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATC26230.20632261210876968No Hit
GACCTAGGACGGCCAGCTGTGTCCCTCCGCCAAATTTCACCCCTGAAGGA24060.18925360454963777No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24040.18909628650761812No Hit
GGGTGGCCGTGTCCCCAGAGTTGGAGCCAGAGAAGCGCTCAGGGATCCCT23220.1826462467848125No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23180.18233161070077322No Hit
CGGCCACCCTGACCATCACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGATGTGGGAGACTGGTA22190.1745443676208006No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGACCTCTGATGTGCTGACT21690.17061141657030937No Hit
CCCCAGGACAGACGGCCAATATTACCTGTGGGACCAACAACATTGGGACT21480.16895957712910306No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGA21390.16825164594001463No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21390.16825164594001463No Hit
GTACCAGTCTCCCACATCTGACAGTAATAGTCGGCCTCATCCCCGGCCTC21020.16534126216265113No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20990.16510528509962166No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20540.16156562915417955No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC20420.16062172090206167No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20300.15967781264994374No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20150.1584979273347964No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20110.15818329125075709No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG20050.15771133712469815No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT19750.15535156649440343No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19240.15133995642290238No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19170.1507893432758336No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18760.1475643234144308No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18340.14426064453201815No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGACCTCTGATGTGCTGACTCAGC17950.141192942712635No Hit
CTGATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG17850.14040635250253675No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17640.13875451306133044No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17450.13725999166214378No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGACCTCTGA17380.136709378515075No Hit
CTATTACTGTCAGATGTGGGAGACTGGTACCGATCCTTCAGGGGTGAAATTTGGCGGAGGGACACAGCTGGCCGT17340.1363947424310357No Hit
GACCATCACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGA17300.1360801063469964No Hit
GACTGGTACCGATCCTTCAGGGGTGAAATTTGGCGGAGGGACACAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGC16880.13277642746458376No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16860.1326191094225641No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATCCA16790.13206849627549536No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16740.13167520117044623No Hit
ATATTACTGTGTGTACTCGGGGACTTATTATTCATACTTTGACTACTGGGGCCAGGGAACCCGGGTCACCGTCTC16420.12915811249813183No Hit
CCTCTCTCACTGCACAGGCTCTTTGACCTCTGATGTGCTGACTCAGCCAC16170.12719163697288624No Hit
GTATATTACTGTGTGTACTCGGGGACTTATTATTCATACTTTGACTACTGGGGCCAGGGAACCCGGGTCACCGT15720.12365198102744412No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTTTGACCT15690.12341600396441467No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15620.12286539081734589No Hit
CTCTTTGACCTCTGATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAATATTAC15530.12215745962825747No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15460.12160684648118869No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTGTGTCCCTCCGCCAAATTTCACCC15250.11995500703998238No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15220.1197190299769529No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15170.11932573487190379No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTGTGTC15050.1183818266197859No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14920.11735925934665817No Hit
GAGTTGGAGCCAGAGAAGCGCTCAGGGATCCCTGAGGGCCGATTTTTATTTTCATATATGACCAACAGAGGGGC14480.1138982624222259No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14460.11374094438020624No Hit
ACCCTGGTGATGGTCAGGGTGGCCGTGTCCCCAGAGTTGGAGCCAGAGAA14320.11263971808606871No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14120.11106653766587221No Hit
GTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGTGATGGTCAGGGTGG14100.11090921962385256No Hit
GGCTCTTTGACCTCTGATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAATATTAC13860.10902140311961678No Hit
CACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTACTGTCAGATGTGGG13680.10760554074143994No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13600.10697626857336133No Hit
ACATTGGGACTGGCAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCC13550.10658297346831222No Hit
GACTATTACTGTCAGATGTGGGAGACTGGTACCGATCCTTCAGGGGTGAA13500.1061896783632631No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13390.10532442913215503No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13300.1046164979430666No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13230.10406588479599783No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12890.1013914780816638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)