FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713237
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT74010.4319892694355772No Hit
GTAATAATCAGCCTCATGCTCGGACCGGAGCCCACTGATGGCCAGGGAGG65930.38482708463569254No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC49640.2897439175082023No Hit
GTAATATACGGCCGTGTCCGCGGCTCTCAGGCTGTTCATTTGCAGATACA48540.2833233230428715No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTCAGGCTGTCATCCCATGCTGCA44970.26248557555084323No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37170.21695772388758824No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35990.21007017709750606No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34450.20108134484604293No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTCAGGCTGTCATCCCATGCTGCACAGTAATAAT34150.19933027362822542No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCCGGGACCC33290.19431053613714858No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31710.1850882277233097No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29930.17469853849759256No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGCTTTTT28980.16915347964117047No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28740.16775262266691648No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28640.16716893226097732No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26110.1524015649907164No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25760.1503586485699293No Hit
GTATATTACTGTGCGAAAGAGGTATACTGTAGTGGTGGTACTTGTTACCCCCCGGCCTACTACGGTATGGACGT25620.1495414820016145No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25590.14936637487983273No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24810.14481358971350722No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24530.14317925657687758No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24420.1425371971303445No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24230.14142818535906007No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCC23850.13921016181649123No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23800.13891831661352164No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23400.136583554989765No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23240.13564965034026233No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22740.13273119831056648No Hit
GCATGGGATGACAGCCTGACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC22290.13010459148384024No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22100.12899557971255582No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22000.12841188930661665No Hit
CCCCTGAGGGCCGCTGATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA21370.1247346397491999No Hit
GTGCTGACTCAGCCACCCTCAGCGTCCGGGACCCCCGGGCAGACGGTCAC21070.1229835685313824No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGCGAAGGGG20570.12006511650168657No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20570.12006511650168657No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20320.11860589048683866No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19560.11416984340170098No Hit
GATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG19530.11399473627991925No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19260.11241877218388348No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19240.11230203410269567No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19170.11189345081853824No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19130.11165997465616256No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18570.10839130838290324No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18440.10763251085518233No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18400.10739903469280665No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18350.10710718948983708No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT18270.10664023716508574No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17900.10448058266311082No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17820.10401363033835948No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17520.102262559120542No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG17390.10150376159282107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCG150.002410616368.60046436
AGGGGGG3600.047.54663570
GGTATCA10650.046.125941
TCTCGTA2700.042.00017538
GTGGTAT2800.041.424111
GGATGGG15200.040.82128570
GCTTGTA857.4578566E-1140.134091
TCGTATG3450.039.9407340
TCTGGAG20550.039.91140770
CGTATGC3600.039.28672441
ACGAGAC3700.037.79535322
GATCTCG2950.037.2070336
ATCTCGT3050.037.14552737
TGCCGTC3750.036.97328645
ATGCCGT3750.036.92727744
TAGGCAT3850.036.37483229
GCTCGGG5100.036.35918870
CGAGACT3850.036.32280723
ATTGTCG501.461382E-435.95071464
ACTAGGC3850.035.46069327
Produced by FastQC (version 0.11.5)