FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524907
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14650.27909705909808785No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13320.2537592373506164No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11600.22099152802305932No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT10010.19070044788886414No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9790.18650922925394403No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9760.1859376994400913No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9570.18231801061902392No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9180.17488812303893833No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9090.1731735335973801No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8450.16098089756852166No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8380.15964732800286527No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8200.15621814911974882No Hit
GAGTATTAGCAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATATGGCGT8090.1541225398022888No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8080.15393202986433788No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG7930.15107438079507418No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7870.1499313211673687No Hit
ATATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC7860.1497408112294178No Hit
GATCTATATGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA7760.14783571184990865No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA7690.14650214228425226No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7630.1453590826565468No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7520.14326347333908673No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7190.1369766453867066No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6890.1312613472481792No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGCAACTGGTTGGCCTGGTATCAGCAGAAA6870.13088032737227737No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6720.12802267830301367No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6560.12497451929579907No Hit
GTTTAATAATTATGTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT6440.12268840004038811No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6430.1224978901024372No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA6240.11887820128136985No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6240.11887820128136985No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6210.1183066714675171No Hit
GTGTATTCCTGTGCGAAACCGATTAGCAGTGGCTGGCAATTCTTTCACTA6060.11544902239825341No Hit
CTCCAGTCCCTTGCCTGGAACCTGGCGGACCCAATGCATGCCATAACTAC6020.11468698264644975No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT5860.11163882363923515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5850.11144831370128423No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC5800.11049576401152966No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5780.11011474413562784No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTA5680.10820964475611869No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCATATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG5650.10763811494226595No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5620.10706658512841323No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT5600.10668556525251141No Hit
GGAATACACAGCCGTGTCCTCAGTGCTCAGGCTGTCCATTTGCAGATACA5540.10554250562480592No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5460.1040184261211986No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC5370.1023038366796404No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5350.10192281680373858No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5310.10116077705193491No Hit
TTCTAAAGTAGACGCCATATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT5280.10058924723808217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTGG200.006465018353.46856755
AATGATT400.006650990353.0787970
CAGGTAT903.6379788E-1241.2672041
GATCAAA700.001188074740.4409870
ATCGTAT350.001371064939.28754439
TTATACA2250.039.005122
ATGCCGT2050.038.99253544
AGGTATC350.001499853838.5764922
TATACAC2200.038.3573073
CCGTCTT2100.038.31214547
CGTATGC2100.037.8488441
TCGTATG2100.037.73109440
TAGGGCT451.13819755E-437.5049255
GTATCAA7650.037.0742841
GCCGTCT2100.036.57779346
TGCCGTC2200.036.4196845
ACGAGAC2350.035.92265322
CACGAGA2300.035.2354421
CTTATAC2400.035.1709141
TACACAT2400.035.1608625
Produced by FastQC (version 0.11.5)