FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302727
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCGTCCAAATCGCTAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGACTTCA49940.38334969644445843No Hit
GTATAGATCAGGAGTTTTGGGGCTTTCCCCGGTTTTTGTTGAAACCAATG49840.38258207590692445No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41120.31564556503396335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37960.29138875604789033No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT30780.23627360145295215No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28380.21785070855213717No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26990.20718078308041518No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26550.20380325271526575No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24560.18852760401834No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23170.17785767854661796No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22990.17647596157905684No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22180.17025823522503178No Hit
GCCTTATATTATTGCGCAAAAGATATGAGAGGAGTGGCGGATGGGGGCTA21350.16388698476349994No Hit
GTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACT21280.16334965038722618No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAACATTATCACCTATTTACATTGGTTTCAACAAAAA21240.1630426021722126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20760.15935802359204962No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20320.1559804932269002No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20280.1556734450118866No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20180.15490582447435264No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20120.15444525215183227No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20090.15421496599057208No Hit
ATATAAGGCCGTGTCCTCAGCTCTCACACTATTCATCTGGAGATATAGGG19570.15022333919539552No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19320.1483042878515606No Hit
GACTTCACTCTCACCATCAGCAGTCTCCAACCTGACGATTTTGCAACTTACTACTGTCAACAGACCCACAGTATT18580.14262389587380933No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAACATTATCAC18330.14070484452997442No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18290.14039779631496085No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17960.13786464854109878No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACCCCA17930.1376343623798386No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17780.13648293157353766No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17610.13517797665972994No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17350.13318216326214163No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17160.13172368424082712No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGAATA17120.13141663602581355No Hit
ACTTACTACTGTCAACAGACCCACAGTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA16370.12565948199430887No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15790.12120728287661191No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15470.11875089715650325No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15380.11806003867272268No Hit
GTCTCCAACCTGACGATTTTGCAACTTACTACTGTCAACAGACCCACAGTATTCCTCGGACGTTCGGCCAAGGGA15360.11790651456521589No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15280.11729241813518872No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15200.11667832170516157No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATACCATAATTAT15020.11529660473760045No Hit
GAGGAATACTGTGGGTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGT14910.11445222214631308No Hit
GTAGTAAGTTGCAAAATCGTCAGGTTGGAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA14780.11345431544751894No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14740.11314726723250536No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC14710.11291698107124516No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14640.1123796466949714No Hit
TCTATACTGCGTCCAAATCGCTAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGACTTCACT14340.11007678508236952No Hit
CAGTATAGATCAGGAGTTTTGGGGCTTTCCCCGGTTTTTGTTGAAACCAATGTAAATAGGTGATAATGTTCTGA13850.10631544444845314No Hit
AGTCAGAACATTATCACCTATTTACATTGGTTTCAACAAAAACCGGGGAAAGCCCCAAAACTCCTGATCTATA13830.10616192034094633No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13830.10616192034094633No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13490.10355201051333088No Hit
CAGTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCTTCAT13370.10263086586829012No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13320.10224705559952316No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13270.10186324533075619No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13200.1013259109544824No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13160.10101886273946882No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13090.10048152836319504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)