FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302727
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60590.46510128369182496No Hit
GTATAGATCAGGAGTTTTGGGGCTTTCCCCGGTTTTTGTTGAAACCAATG48450.3719121504352025No Hit
GATCTATACTGCGTCCAAATCGCTAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGACTTCA48350.37114452989766855No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42230.3241661530005903No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30500.23412426394785707No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27160.2084857379942229No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26150.20073277056512992No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25580.19635733350118634No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24470.18783674553455942No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22210.170488521386292No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21290.16342641244097958No Hit
GCCTTATATTATTGCGCAAAAGATATGAGAGGAGTGGCGGATGGGGGCTA21220.16288907806470582No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20920.16058621645210394No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAACATTATCACCTATTTACATTGGTTTCAACAAAAA20060.1539846798293119No Hit
GTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACT19940.15306353518427115No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19840.15229591464673717No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19370.14868809812032757No Hit
ATATAAGGCCGTGTCCTCAGCTCTCACACTATTCATCTGGAGATATAGGG19040.14615495034646553No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18920.14523380570142477No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18810.14438942311013742No Hit
GACTTCACTCTCACCATCAGCAGTCTCCAACCTGACGATTTTGCAACTTACTACTGTCAACAGACCCACAGTATT18370.14101189274498802No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18030.13840198291737255No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACCCCA17890.13732731416482502No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17630.1353315007672367No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17520.13448711817594938No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAACATTATCAC17190.1319539704020873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16790.1288834882519515No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16430.12612005431682924No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16420.12604329226307584No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGAATA16340.12542919583304868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16230.12458481324176132No Hit
ACTTACTACTGTCAACAGACCCACAGTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA16040.12312633422044679No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16020.12297281011294No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15770.12105375876910512No Hit
GTAGTAAGTTGCAAAATCGTCAGGTTGGAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA15490.11890442126401005No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15080.11575717706012081No Hit
GGGTTGGGACTGAACTGGATTTTCCTTTTGGCTATTTTAAAAGATGTCCAGTGTGAAGTGCAGCTGGTGGAGT15020.11529660473760045No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14750.11322402928625876No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14590.11199583642620442No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14370.1103070712436297No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14340.11007678508236952No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14240.10930916454483557No Hit
GTCTCCAACCTGACGATTTTGCAACTTACTACTGTCAACAGACCCACAGTATTCCTCGGACGTTCGGCCAAGGGA14010.10754363730850747No Hit
AGTCAGAACATTATCACCTATTTACATTGGTTTCAACAAAAACCGGGGAAAGCCCCAAAACTCCTGATCTATA13960.10715982703974047No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13950.10708306498598708No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC13790.10585487212593275No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13500.10362877256708428No Hit
TCTATACTGCGTCCAAATCGCTAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGACTTCACT13430.10309143819081051No Hit
GAGGAATACTGTGGGTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGT13390.10278438997579692No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATACCATAATTAT13310.10217029354576976No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13310.10217029354576976No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13240.10163295916949598No Hit
CAGTATAGATCAGGAGTTTTGGGGCTTTCCCCGGTTTTTGTTGAAACCAATGTAAATAGGTGATAATGTTCTGA13210.1014026730082358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)