FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248925
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT37801.5185296776137391No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC7670.3081249372300894No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC7560.3037059355227478No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT7260.29165411268454355No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA7140.2868333835492618No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT6180.24826755046700816No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA4660.18720498142010647No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4480.17997388771718387No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT4080.1639047905995782No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT3920.1574771517525359No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT3800.15265642261725418No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3770.15145124033343377No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA3750.15064778547755348No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACT3750.15064778547755348No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC3580.14381841920257105No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA3500.1406045997790499No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG3500.1406045997790499No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT3470.1393994174952295No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA3360.13498041578788791No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATGTCGTATGCCGT3360.13498041578788791No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC3330.13377523350406748No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTA3310.1329717786481872No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG3260.13096314150848648No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT3250.13056141408054636TruSeq Adapter, Index 10 (95% over 21bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG3210.12895450436878578No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT3190.12815104951290548No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT3070.12333032037762379No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGC2920.11730440895852165No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT2870.11529577181882092No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA2760.11087677011147937No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG2610.10485085869237723No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT2580.10364567640855679No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2580.10364567640855679No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA2550.10244049412473637No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCG2550.10244049412473637No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA2510.1008335844129758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.1008335844129758No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACA2510.1008335844129758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)