FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068864
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38930.3642184599724567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32170.3009737440871804No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25740.2408164181785522No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT24450.22874753008801868No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24270.22706349919166519No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22490.21041030477216932No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTCA22450.21003607568409075No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22130.20704224297946233No Hit
GTGTTAGTACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA21830.20423552481887314No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA21330.19955766121789115No Hit
ATATAAGATCTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAACCAGACGAACTGTGGCTGCACCAT20510.1918859649122807No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20140.18842434584755405No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20020.18730165858331838No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19530.18271735225435604No Hit
CTGGTATCCCAGCCAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTCACT19180.17944284773366864No Hit
GTCTGGGTCTCCAGGGGAAGGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTAGTACCAACTTAGCCTGGTA18170.16999356325968504No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18090.16924510508352794No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17940.16784174600323334No Hit
GTCCCAGACCCACTGCCACTAAACCTGGCTGGGATACCAGTGGCCCTGGT17670.16531569965870307No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGCAGTCTGAAGATT17540.16409945512244775No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17170.1606378360577211No Hit
GTCTCCAGCCACCCTGTCTGGGTCTCCAGGGGAAGGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTAGTAC16600.15530507155260162No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16190.15146922339979643No Hit
GTTTATTACTGTCAGCAATATAAGATCTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAACCAGACGAA16050.15015942159152146No Hit
ATCTTATATTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15980.14950452068738398No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15640.14632357343871624No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15560.14557511526255915No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15460.14463954254236275No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15310.14323618346206815No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15260.14276839710196995No Hit
GTCTCTGGTGGCTCCCTCAGTCCTTATTACTGGAGCTGGATCCGGCAGCC15230.14248772528591103No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC15070.1409908089335968No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14650.1370614035087719No Hit
GACTTCACTCTCACCATCAGCAGACTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAATATAAGATCTGG14120.13210286809173102No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13960.1306059517394168No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGGGTCTCCAGG13830.12938970720316148No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGGTTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGCCAGAT13750.12864124902700438No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGTCTGCTGATGGTGAGAGTGA13310.12452472905814024No Hit
GTGCAGCCACAGTTCGTCTGGTTTCCACCTTGGTCCCTTGGCCGAACGTT13270.12415049997006168No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12790.11965975091311896No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12270.11479477276809771No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12190.1140463145919406No Hit
GTCAGAGTGTTAGTACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG12040.11264295551164602No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTAAACCTGGCTGGGATACCA11870.11105248188731213No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11860.11095892461529248No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11660.10908777917489972No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11560.10815220645470332No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11510.10768442009460512No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11280.1055326028381534No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10980.1027258846775642No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10730.10038695287707324No Hit
GGGCCAGTCAGAGTGTTAGTACCAACTTAGCCTGGTACCAGCAGAAACCT10720.1002933956050536No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10690.10001272378899466No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGTCG352.3737019E-548.92482436
GGTATCA10800.043.945981
GTCGTAT551.501212E-743.7904639
TCGTATG5700.043.53771640
CGTATGC5750.043.2414341
TGCCGTC5850.042.82275845
CCGTCTT5800.042.71924647
ATCTCGT5150.042.6223137
AGACGCG6150.042.48913225
CGCGTAG6000.042.47786328
ACGCGTA6000.041.88398727
TATACAC6650.041.787323
GCGTAGT6150.041.4612729
ATGCCGT6050.041.3039744
GCCGTCT6000.041.25155346
TGTCGTA503.9479637E-641.2372238
GACGCGT6100.041.1838526
GTATGCC6150.041.05507742
CTTATAC6750.040.681421
GTAATCT5400.040.5090534
Produced by FastQC (version 0.11.5)