FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068864
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56150.5253240823902761No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40380.3777842644153045No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27340.2557855817016945No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25440.238009700017963No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25340.2370741272977666No Hit
GTGTTAGTACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA22790.21321702293275852No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22570.21115876294832644No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20890.19544114124902698No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20530.19207307945632No Hit
ATATAAGATCTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAACCAGACGAACTGTGGCTGCACCAT20480.1916052930962218No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTCA20420.19104394946410394No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA19390.18140755044608106No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGCAGTCTGAAGATT19080.17850727501347224No Hit
CTGGTATCCCAGCCAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTCACT18560.17364229686845098No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17850.1669997305550566No Hit
GTCTGGGTCTCCAGGGGAAGGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTAGTACCAACTTAGCCTGGTA17640.16503502784264415No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17300.1618540805939764No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17140.16035716424166216No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16610.15539862882462127No Hit
GTCCCAGACCCACTGCCACTAAACCTGGCTGGGATACCAGTGGCCCTGGT16610.15539862882462127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16580.15511795700856237No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16400.15343392611220885No Hit
ATCTTATATTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15940.14913029159930544No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15810.1479140470630501No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15540.14538800071851984No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15460.14463954254236275No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15000.1403359080294593No Hit
GTCTCCAGCCACCCTGTCTGGGTCTCCAGGGGAAGGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTAGTAC14950.1398681216693611No Hit
GACTTCACTCTCACCATCAGCAGACTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAATATAAGATCTGG14950.1398681216693611No Hit
GTTTATTACTGTCAGCAATATAAGATCTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAACCAGACGAA14910.13949389258128256No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14270.13350622717202562No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14110.13200931081971137No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14080.13172863900365248No Hit
GTCTCTGGTGGCTCCCTCAGTCCTTATTACTGGAGCTGGATCCGGCAGCC14010.131073738099515No Hit
GTGCAGCCACAGTTCGTCTGGTTTCCACCTTGGTCCCTTGGCCGAACGTT13830.12938970720316148No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13290.12433761451410094No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC13150.123027812705826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12950.1211566672654332No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12570.11760149092868691No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGGTTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGCCAGAT12560.11750793365666726No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGGGTCTCCAGG12560.11750793365666726No Hit
GTCAGAGTGTTAGTACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG12290.114981887312137No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGTCTGCTGATGGTGAGAGTGA12180.11395275731992097No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12170.11385920004790132No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTAAACCTGGCTGGGATACCA12160.11376564277588168No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11750.10992979462307648No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11560.10815220645470332No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11320.10590683192623196No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11210.10487770193401594No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11110.10394212921381954No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10960.10253877013352494No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10790.10094829650919106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.042.900851
CACTGAG10050.036.79630770
GTATCAA26300.035.3570671
TCCGGAA2950.032.592870
TGTTAGA9550.030.97825270
ATCAACG31650.029.9160253
TATCAAC31650.029.699562
GCTTGTT1000.005507612629.58423270
TCAACGC32600.029.0442394
CGGGATT704.397682E-529.00125717
CAACGCA32650.028.9970475
AACGCAG32850.028.8205056
ACGCAGA33550.028.11847
CGCAGAG33500.028.0594358
GCAGAGT34650.027.12699
AGGTACG500.00853258927.0501022
TGGATTG9850.027.0312870
AGAGTAC34800.026.90400111
ATATAAC659.73316E-426.0109311
TGTACCG700.0010382525.72443461
Produced by FastQC (version 0.11.5)