FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378649
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37030.2685962852038481No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29860.21658884893834474No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29840.21644377938111878No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28980.21020578842040286No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA28250.20491074958165567No Hit
GTGTGGTGGTCTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAG28160.20425793657413888No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27110.19664178481977646No Hit
GCCCCACTCTGTGTCGGAGTCTCTGGGGATGACGGCAACCATCTCCTGCACCCGCATCAGTGGCAACATTGCCAG26940.19540869358335589No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT26860.19482841535445208No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT25670.18619677669950802No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25180.1826425725474722No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25060.1817721552041165No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24720.17930597273127533No Hit
GACTACTACTGTCAGTCTTATGAAAGCAGTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG21810.15819835215489947No Hit
GTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC21440.15551456534621938No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21380.15507935667454154No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21110.1531209176519912No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAACTGCTTTCATAAGACTGACAGTAGTAGTCAG21070.1528307785375393No Hit
GTGTCGGAGTCTCTGGGGATGACGGCAACCATCTCCTGCACCCGCATCAGTGGCAACATTGCCAGCAACTTTGTG20540.14898643527105157No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19350.14035479661610753No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18710.13571257078487708No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18200.13201329707561532No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18050.13092527539642068No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17940.13012739283167796No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17920.12998232327445203No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17850.1294745798241612No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17810.1291844407097093No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17740.12867669725941847No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17550.12729853646577194No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17200.12475981921431778No Hit
CTACTACTGTCAGTCTTATGAAAGCAGTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC17080.12388940187096208No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15990.11598311100214774No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGAAG15880.11518522843740502No Hit
GTACCAGCACCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAAGATA15870.11511269365879204No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15840.11489508932295313No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15470.11221130251427303No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG15160.1099627243772708No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14530.10539303332465334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.10539303332465334No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14370.10423247686684572No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14240.10328952474487704No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTCGA14220.10314445518765109No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13980.10140362050093969No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13810.10017052926451911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG7450.052.08288670
CGGGCTA200.00802445250.62966517
TCGTATG6100.046.85770440
TCGACGT8450.046.51276829
ACTCGAC8800.044.97743627
ATCTCGT4750.044.71679337
CGACGTC8500.044.70493330
CGTATGC6600.044.48478341
TCTCGTA4650.044.28646538
ACGTCAT7800.044.0494532
GACGTCA8450.043.82883531
GGTATCA23300.043.624371
ATGCCGT6700.043.57376544
CACGAGA9400.043.09689321
GCCGTCT6550.042.6402846
GACTCGA9300.042.53146426
CCCACGA9450.042.50391819
TCTCCGA9700.042.1028711
AGACTCG9500.041.97363325
ATCTCCG9750.041.88695510
Produced by FastQC (version 0.11.5)