FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378649
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82110.5955830671911415No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36330.26351885070093983No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33640.24400699525404945No Hit
GTGTGGTGGTCTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAG30800.22340711812796438No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT27220.1974396673845192No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26780.19424813712554828No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26730.1938854632324834No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA26450.19185448943132008No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26280.19062139819489948No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25680.18626931147812098No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23830.1728503774347205No Hit
GCCCCACTCTGTGTCGGAGTCTCTGGGGATGACGGCAACCATCTCCTGCACCCGCATCAGTGGCAACATTGCCAG22770.16516169090174512No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22320.1618976258641612No Hit
GTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC21950.15921383905548112No Hit
GTGTCGGAGTCTCTGGGGATGACGGCAACCATCTCCTGCACCCGCATCAGTGGCAACATTGCCAGCAACTTTGTG21790.15805328259767354No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21330.15471668278147663No Hit
GACTACTACTGTCAGTCTTATGAAAGCAGTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG20310.14731813536295316No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18900.1370907315785236No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAACTGCTTTCATAAGACTGACAGTAGTAGTCAG18890.13701819679991062No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17420.12635558434380326No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17140.12432461054263994No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17100.12403447142818803No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16810.12193096284841175No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16790.12178589329118579No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16590.12033519771892627No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16400.11895703692527974No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16390.11888450214666677No Hit
CTACTACTGTCAGTCTTATGAAAGCAGTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC16040.11634578489521263No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15860.11504015888017906No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGAAG15700.11387960242237147No Hit
GTACCAGCACCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAAGATA15270.1107606069420135No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG15220.11039793304894864No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15080.10938244614836698No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14580.10575570721771822No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14350.10408740730961977No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCTGGGGATGACGGCAAC14120.10241910740152135No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14110.10234657262290837No Hit
GTGCTGGTACCACTGCACAAAGTTGCTGGCAATGTTGCCACTGATGCGGGTGCAGGAGATGGTTGCCGTCATCCC14110.10234657262290837No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC14050.10191136395123052No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13910.10089587705064886No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTCGA13880.10067827271480992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)