FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779020
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT72440.407190475655136No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA67230.3779046890984924No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61500.3456959449584603No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC60370.3393441332868658No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC58380.32815819945812863No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG56710.3187710087576306No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG55960.31455520455082014No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC53160.2988162021787276No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAAATGTAAGGGGTGGTGT51720.29072185810165146No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC51330.28852963991411No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACGTT48590.2731279018785624No Hit
GTATATGAACTGCAATAATAATCAGCCTCGTCTTCAGCCTGGAGCCCAGA48340.2717226338096255No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC48190.2708794729682634No Hit
GTTCATATACAATGAACACCACCCCTTACATTTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGG48010.2698676799586289No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAAATGTAAGGGGTGGTGTTCATTGTATATGAA45380.25508425987341343No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAAATGTAAGGGGTGGTG44390.2495193983204236No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT39740.22338141223819855No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT38340.21551191105215228No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG38310.21534327888387989No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC37800.21247653202324876No Hit
CTCCTGCACTGGAACCAGTAGTGACGTTGGTCGTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC37650.21163337118188666No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37070.20837314926195322No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG35460.199323222898No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACGTTGGTCGTTATAACTTTGTCTCCTGGTACCAACAACA35450.19926701217524256No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG35400.19898595856145518No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC34920.19628784386909645No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT31860.17908736270530967No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG30120.16930669694550932No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG27890.1567717057705928No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC27510.1546356983058088No Hit
TCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTATTGCAGTTCATAT27250.15317421951411453No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA26760.1504198940989983No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC25440.14300007869501186No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24190.1359737383503277No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG24120.13558026329102538No Hit
GTAGTGACGTTGGTCGTTATAACTTTGTCTCCTGGTACCAACAACACCCA23470.13192656631178964No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23210.13046508752009534No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA23000.1292846623421884No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC22860.12849771222358378No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC22260.12512506885813537No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG22090.12416948657125834No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22000.12366359006644106No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG21890.12304527211610887No Hit
GTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTG21750.12225832199750425No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT21500.1208530539285674No Hit
GTCTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAAGTTATAA21140.11882946790929838No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21020.11815493923620869No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC20960.11781767489966385No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20470.11506334948454768No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG19980.1123090240694315No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC19910.11191554901012918No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG19620.11028543805016244No Hit
GACTTGGACCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTGAC19530.1097795415453452No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19490.10955469865431529No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT19280.10837427347640835No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG19250.10820564130813594No Hit
GACCATCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTATTGCAGTTCATATACAATGAACACCACCCCTTA19230.108093219862621No Hit
GATTATTATTGCAGTTCATATACAATGAACACCACCCCTTACATTTTCGG19190.1078683769715911No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG18960.1065755303481692No Hit
GGCAAAGCCCCCAGACTCATGATTTATGACGTCAGTAATCGGCCCTCAGG18880.10612584456610942No Hit
TTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAGAC18790.10561994806129217No Hit
CAATGAACACCACCCCTTACATTTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA18580.10443952288338523No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18370.1032590977054783No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT18170.10213488325032884No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17920.10072961518139201No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17850.10033614012208969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT200.00741381651.65416339
GGTATCA14850.047.399221
TGCCGTT750.00187255436.8750170
TGGTATC3950.035.449462
GTGGTAT4100.035.0022431
CGTATGC1800.034.51239841
GTATCAA32450.034.4320951
TCGTATG1850.033.54152340
AGGGGGG4350.033.3782570
CGAGACG3000.033.01782223
ATCTCGT2000.032.6578537
ATGCCGT2400.031.74184244
TATACAC3650.029.9419443
ATGAATC3350.029.6691133
GCCGTCT2400.028.90977146
CGCTCAT3550.028.89052428
TCTCGTA2150.028.80411138
ACGCTCA3450.028.7313427
TATCAAC38950.028.4969752
AATCTCG2900.028.4287636
Produced by FastQC (version 0.11.5)