FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779020
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT69190.38892199075895717No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC64280.3613225258850378No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA59560.33479106474351045No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG53780.30230126698969095No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC53210.299097255792515No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG52610.2957246124270666No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC52060.2926330226754056No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51410.2889793256961698No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC51330.28852963991411No Hit
GTTCATATACAATGAACACCACCCCTTACATTTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGG47470.2668323009297254No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAAATGTAAGGGGTGGTGT46190.2596373284167688No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC45640.25654573866510777No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACGTT44450.24985666265696843No Hit
GTATATGAACTGCAATAATAATCAGCCTCGTCTTCAGCCTGGAGCCCAGA44020.24743960157839712No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAAATGTAAGGGGTGGTGTTCATTGTATATGAA43690.2455846477274005No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC41840.23518566401726795No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAAATGTAAGGGGTGGTG40200.2259671054850423No Hit
CTCCTGCACTGGAACCAGTAGTGACGTTGGTCGTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC38840.218322447190026No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37580.21123989612258434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG37350.20994704949916246No Hit
TCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTATTGCAGTTCATAT36470.20500050589650481No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT36390.20455082011444503No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT36370.2044383986689301No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35710.20072849096693687No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACGTTGGTCGTTATAACTTTGTCTCCTGGTACCAACAACA34810.19566952591876427No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG34750.19533226158221945No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG33090.18600128160447887No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC33000.18549538509966162No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG30220.16986880417308406No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT29590.16632752863936323No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG29100.16357320322424707No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA27220.15300558734584208No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC26260.14760935796112468No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC25410.14283144652673946No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC25250.14193207496261986No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC24740.13906532810198874No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24250.13631100268687255No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA22550.1267551798181021No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG22530.12664275837258715No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC22060.1240008544029859No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG21550.12113410754235478No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21380.12017852525547773No Hit
GTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTG21120.11871704646378342No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21100.11860462501826848No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC20980.1179300963451788No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20800.11691830333554429No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT20540.11545682454384999No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20350.11438882081145799No Hit
GTCTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAAGTTATAA20300.11410776719767064No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20220.11365808141561085No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG20010.11247765623770391No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG19890.11180312756461422No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT19290.10843048419916584No Hit
GACCATCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTATTGCAGTTCATATACAATGAACACCACCCCTTA19270.10831806275365088No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19060.10713763757574395No Hit
GATTATTATTGCAGTTCATATACAATGAACACCACCCCTTACATTTTCGG18790.10561994806129217No Hit
GACTTGGACCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTGAC18360.10320288698272083No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18130.10191004035929893No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA18080.10162898674551156No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT17890.10056098301311958No Hit
GTAGTGACGTTGGTCGTTATAACTTTGTCTCCTGGTACCAACAACACCCA17800.10005508650830232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAT150.002320895469.25989544
GGTATCA14350.041.3774151
GTATCAA33000.035.572181
TGGTATC4900.035.5143172
GTGGTAT5000.035.4894561
ACGTGAT1102.6080725E-432.5731870
GAATACG959.817086E-932.320279
ACGTACC1001.61981E-830.70080213
TATCAAC40250.028.9927642
ATCAACG40550.028.6091653
TCAACGC40350.028.5794374
CAACGCA40950.028.0765845
CGTACCA1104.0832674E-827.91531614
TACGTAC1104.0921805E-827.90903912
AACGCAG41400.027.8538136
TACGTGA807.206625E-427.38525669
ACGCAGA43350.026.7582747
CGCAGAG43850.026.0641468
AGAGTAC43900.025.87466611
ATACGTA1209.512769E-825.58328411
Produced by FastQC (version 0.11.5)