FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076940
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34860.3236949133656471No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34030.315987891618846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23830.22127509424851896No Hit
GTAATAAATAGCCGTGTCGTCGACTCTCAGACTGTTCATGTCCAGATACA22050.20474678255055992No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21830.2027039575092391No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21610.20066113246791834No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21340.19815402900811557No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20520.19053986294501085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20410.18951845042435048No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT20320.18868274927108286No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20320.18868274927108286No Hit
CTTTTCTACAGCTCCAACAATAGCAGCTACTTAGCTTGGTACCAACAGAA19760.18348283098408455No Hit
ATTTATTACTGTGCAAGAGCCATTCCCGGCGGGAGCTACCTCCCCTACTGGGGCCAGGGAGCCCTGGTCACCGT18830.17484725240031943No Hit
GTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG18430.17113302505246344No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18090.1679759318067859No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17750.16481883856110832No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17620.16361171467305513No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17260.16026891005998478No Hit
ATTTTAGTACTCCGTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA16670.15479042472189722No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCAGGGACAGATTTCACTCTCAC15870.1473619700261853No Hit
GTACTAAAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCCGCAGGCTGCTGATGGTGAGAGTGAAAT15660.14541200066856091No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14970.13900495849350938No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14610.135662153880439No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14270.13250506063476147No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13960.12962653444017308No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13650.12674800824558471No Hit
AAACTATACGGAGTACTAAAATATTGCTGACAATAATAAACTGCCACATC13430.12470518320426394No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13160.12219807974446116No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12860.11941240923356918No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12860.11941240923356918No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12620.1171838728248556No Hit
GTAGAAAAGACTGTGGCTGGACTTGCAGTTGATGGTGGTCCTCTCGCCCAGAGACACAGCCAGGGAGTCGGGAGA12570.1167195944063736No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12480.11588389325310604No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12400.11514104778353484No Hit
GATTTCACTCTCACCATCAGCAGCCTGCGGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTTTAGTACT12350.11467676936505285No Hit
GTCTGAGAGTCGACGACACGGCTATTTATTACTGTGCAAGAGCCATTCCC12330.11449105799766005No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12150.11281965569112486No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11910.11059111928241128No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11880.11031255223132208No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11460.10641261351607331No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTGCCTACTGGATGCACTGGGTCCGCCAAGCTCCAGGTAAGGGG11440.10622690214868052No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11430.10613404646498412No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11380.10566976804650212No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11240.10436978847475253No Hit
GTCAGGGACTCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTATTTTAGGAGGCTGTCCTGATTTCTGTTGGTA11220.10418407710735973No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11190.10390551005627054No Hit
GATCAGGACTGCACACAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGG10950.10167697364755696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCG252.7056722E-454.6155934
CGTGACT305.3265435E-447.61046257
CGTATGG306.291941E-446.02856441
CGTATGC4750.045.7863141
TCGTATG4900.045.72004740
GTTAGCC452.1363685E-645.0882453
ATGCCGT4850.045.07513444
GGTATCA9700.044.650371
ATCTCGT4500.043.4061937
TATCTCG4500.043.3572336
ACGAGAC5400.043.23164722
GCCGTCT5000.043.19556446
AGCCTAT5250.042.82995232
TATACAC5700.042.7151833
GCCTATC4850.042.17912333
CTTATAC5750.041.7808531
GAGCCTA5650.041.5678431
CGAGACC5700.041.5556623
GAGACCG5550.041.4651724
CACGAGA5550.041.44783821
Produced by FastQC (version 0.11.5)