FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076940
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45880.4260218767990789No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34820.3233234906308615No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26370.2448604379074043No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24360.2261964454844281No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23880.22173937266700094No Hit
GTAATAAATAGCCGTGTCGTCGACTCTCAGACTGTTCATGTCCAGATACA19510.18116143889167458No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19360.17976860363622857No Hit
ATTTATTACTGTGCAAGAGCCATTCCCGGCGGGAGCTACCTCCCCTACTGGGGCCAGGGAGCCCTGGTCACCGT19230.17856147974817538No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18960.17605437628837262No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18950.1759615206046762No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18660.17326870577748063No Hit
CTTTTCTACAGCTCCAACAATAGCAGCTACTTAGCTTGGTACCAACAGAA18190.1689044886437499No Hit
GTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG17350.16110461121325237No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16860.1565546827121288No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16780.15581183724255762No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15840.14708340297509612No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15760.1463405575055249No Hit
ATTTTAGTACTCCGTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15710.14587627908704293No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15670.14550485635225732No Hit
GTACTAAAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCCGCAGGCTGCTGATGGTGAGAGTGAAAT15110.14030493806525898No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCAGGGACAGATTTCACTCTCAC14820.1376121232380634No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14730.1367764220847958No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14370.13343361747172544No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13920.1292551117053875No Hit
AAACTATACGGAGTACTAAAATATTGCTGACAATAATAAACTGCCACATC13700.1272122866640667No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13650.12674800824558471No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12930.12006239901944397No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12760.1184838523966052No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12260.11384106821178525No Hit
GTCTGAGAGTCGACGACACGGCTATTTATTACTGTGCAAGAGCCATTCCC12260.11384106821178525No Hit
GATTTCACTCTCACCATCAGCAGCCTGCGGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTTTAGTACT12230.11356250116069605No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTGCCTACTGGATGCACTGGGTCCGCCAAGCTCCAGGTAAGGGG12020.11161253180307167No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11970.11114825338458967No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11840.10994112949653649No Hit
GAGTGAAATCTGTCCCTGACCCGCTGCCACTGAATCGGTCAGGGACTCCG11800.10956970676175089No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11700.10864114992478689No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11350.10539120099541292No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11070.10279124185191375No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTA11040.10251267480082456No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11000.10214125206603895No Hit
GGCCAAAACTATACGGAGTACTAAAATATTGCTGACAATAATAAACTGCC10880.10102698386168217No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10850.10074841681059296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)