FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777881
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCCCCTCACACTTTTGGCCAGGGGACCAGCCTGCAGATCAAACGAACTGTGGCTGCACCAT117350.660055425531855No Hit
GTTTTATACACCTCCAATGATAGGAACTACTTAGCTTGGTACCAGCAGAA93450.5256257308560022No Hit
CTATAATATTGCTGACAGTAATAAAGTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC87220.49058401546560204No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82880.46617293283408734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC81850.46037951921416564No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT63820.358966657498449No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58360.32825594063944663No Hit
GCTTAGGAGGCTGTCCTACTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCATTGGAGGTGTATAAAACACT52110.2931017317806985No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48720.2740340888957135No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG48430.2724029336046676No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG46150.25957867821299624No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46140.25952243147882226No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41450.23314271315121765No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41260.23207402520191173No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41210.23179279153104174No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40920.2301616362399958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40360.22701181912625198No Hit
AAGTAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA39600.22273706732902823No Hit
TTATTACTGTCAGCAATATTATAGTGCCCCTCACACTTTTGGCCAGGGGA38870.21863105573432642No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38060.21407507026623265No Hit
CTTTTGGCCAGGGGACCAGCCTGCAGATCAAACGAACTGTGGCTGCACCA35830.20153204854543133No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35670.20063210079864735No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35230.1981572444949915No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT33360.18763910520445407No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32360.18201443178705437No Hit
CTTTATTACTGTCAGCAATATTATAGTGCCCCTCACACTTTTGGCCAGGGGACCAGCCTGCAGATCAAACGAA31100.17492734328113074No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30370.17082133168642896No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29490.1658716190791172No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA29240.1644654507247673No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA28960.1628905421678954No Hit
CCTCCAATGATAGGAACTACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGCTGCTCATTTACTGGG27710.15585970039614575No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27600.15524098632023178No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTT27010.15192242900396596No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26930.15147245513057397No Hit
GAACTACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGCTGC26660.14995379330787606No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCACTTTATTACTGTCAGCAATATTATAGTGCC26250.14764767720674218No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25640.14421662642212837No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC24970.14044809523247057No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24790.1394356540173386No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24510.1378607454604667No Hit
CAGTAATAAAGTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA24220.1362295901694208No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23770.1336984871315909No Hit
GTCCTACTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCATTGGAGGTG23690.13324851325819895No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23670.13313601978985096No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23560.132517305713937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23560.132517305713937No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23500.132179825308893No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA23350.13133612429628305No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23090.12987370920775912No Hit
GGCCAAAAGTGTGAGGGGCACTATAATATTGCTGACAGTAATAAAGTGCC22570.12694887903071128No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22030.12391155538531544No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22030.12391155538531544No Hit
CTACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC21950.12346158151192346No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21810.1226741272334875No Hit
GGATCTCTGGTGCGTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG21790.1225616337651395No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTACTTTCTGCTGGTACCAAGCTA21790.1225616337651395No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21640.12171793275252957No Hit
GTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG21470.1207617382715716No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21330.11997428399313564No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20960.11789315482869775No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20930.11772441462617578No Hit
GTAATAAAGTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA20780.1168807136135658No Hit
GTGCAGCCACAGTTCGTTTGATCTGCAGGCTGGTCCCCTGGCCAAAAGTG20780.1168807136135658No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20640.11609325933512986No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20590.11581202566425988No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20380.11463084424660594No Hit
GACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCA20120.113168429158082No Hit
GGCTGAGGATGTGGCACTTTATTACTGTCAGCAATATTATAGTGCCCCTCACACTTTTGGCCAGGGGACCAGC20090.11299968895556002No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20030.11266220855051604No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19910.11198724774042806No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTACTTTCTGCTGGTA18940.10653131452555036No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACACCTCCAATGATAGGAACTA18900.10630632758885437No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18670.10501265270285243No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18600.10461892556363446No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18560.10439393862693848No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18360.10326900394345853No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18280.10281903007006656No Hit
ACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGCTGCTCATT18040.10146910844989063No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18040.10146910844989063No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17980.10113162804484666No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17900.10068165417145467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG7950.048.93980440
CGTATGC8550.048.8250941
ATGCCGT8400.048.34030544
CGCGTAT507.172275E-848.10557639
GTATGCC8900.047.37434442
GCCGTCT7450.047.24897446
TCGACGT11000.046.84785529
CGTCTTC7600.046.02355248
CTCGACG11400.045.4732128
ATCTCGT6800.045.30676737
CGACGTC11200.045.14396730
CCGTCTT7900.045.07989547
GGTATCA22250.044.9324341
ACGAGAC11800.044.38324422
CACGAGA12000.043.92138721
CCCACGA12100.043.81912219
GACGTCA11400.043.81113431
CTTATAC12000.043.626331
AGACTCG11900.043.4864725
CATCTCG7200.043.20848536
Produced by FastQC (version 0.11.5)